Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate

Francisco José Pérez-Llarena, Ana Fernández, Laura Zamorano, Frédéric Kerff, Alejandro Beceiro, Belén Aracil, Emilia Cercenado, Elisenda Miro, Antonio Oliver, Jesús Oteo, Ferran Navarro, Germań Bou

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9 Citations (Scopus)

Abstract

An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β- lactamase was discovered in Madrid, Spain. The blaIMP-28 gene is part of a chromosomally located class I integron. The IMP-28 kcat/K m values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)4540-4543
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number8
DOIs
Publication statusPublished - 1 Aug 2012

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