TY - JOUR
T1 - Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate
AU - Pérez-Llarena, Francisco José
AU - Fernández, Ana
AU - Zamorano, Laura
AU - Kerff, Frédéric
AU - Beceiro, Alejandro
AU - Aracil, Belén
AU - Cercenado, Emilia
AU - Miro, Elisenda
AU - Oliver, Antonio
AU - Oteo, Jesús
AU - Navarro, Ferran
AU - Bou, Germań
PY - 2012/8/1
Y1 - 2012/8/1
N2 - An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β- lactamase was discovered in Madrid, Spain. The blaIMP-28 gene is part of a chromosomally located class I integron. The IMP-28 kcat/K m values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
AB - An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β- lactamase was discovered in Madrid, Spain. The blaIMP-28 gene is part of a chromosomally located class I integron. The IMP-28 kcat/K m values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior. Copyright © 2012, American Society for Microbiology. All Rights Reserved.
U2 - 10.1128/AAC.00776-12
DO - 10.1128/AAC.00776-12
M3 - Article
SN - 0066-4804
VL - 56
SP - 4540
EP - 4543
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 8
ER -