Characterization and expression of NADPH oxidase in LPS-, poly(I:C)- and zymosan-stimulated trout (Oncorhynchus mykiss W.) macrophages

Sebastian Boltaña, Carmen Doñate, Frederick W. Goetz, Simon MacKenzie, Joan C. Balasch, Lluis Tort

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

In vertebrates, the generation of superoxide reactive oxygen species (ROS) via activation of the Nox/Duox family of NADPH oxidases is a prototypical feature of the pathogen-induced defensive responses of activated professional phagocytes. To understand the role of the rainbow trout (Oncorhynchus mykiss) Phox oxidase from a phylogenetic and functional perspective we describe the cloning, sequencing and expression analysis of multiple NADPH components in cultured macrophages. Phylogenetic analyses support the notion of the emergence of Phox-related components before the diversification of basal euteleosts and add to the limited collection of teleost NADPH oxidases. Expression studies using lipopolysaccharide, polyinosine-polycytidylic acid and zymosan to mimic the onset of inflammatory responses in trout macrophages suggest differences in regulation of the NADPH complex throughout the maturation/differentiation period of culture and between different treatments. © 2008 Elsevier Ltd.
Original languageEnglish
Pages (from-to)651-661
JournalFish and Shellfish Immunology
Volume26
DOIs
Publication statusPublished - 1 Jan 2009

Keywords

  • LPS
  • Macrophages
  • NADPH oxidase
  • Poly(I:C)
  • Trout
  • Zymosan

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