Abstract
In vertebrates, the generation of superoxide reactive oxygen species (ROS) via activation of the Nox/Duox family of NADPH oxidases is a prototypical feature of the pathogen-induced defensive responses of activated professional phagocytes. To understand the role of the rainbow trout (Oncorhynchus mykiss) Phox oxidase from a phylogenetic and functional perspective we describe the cloning, sequencing and expression analysis of multiple NADPH components in cultured macrophages. Phylogenetic analyses support the notion of the emergence of Phox-related components before the diversification of basal euteleosts and add to the limited collection of teleost NADPH oxidases. Expression studies using lipopolysaccharide, polyinosine-polycytidylic acid and zymosan to mimic the onset of inflammatory responses in trout macrophages suggest differences in regulation of the NADPH complex throughout the maturation/differentiation period of culture and between different treatments. © 2008 Elsevier Ltd.
Original language | English |
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Pages (from-to) | 651-661 |
Journal | Fish and Shellfish Immunology |
Volume | 26 |
DOIs | |
Publication status | Published - 1 Jan 2009 |
Keywords
- LPS
- Macrophages
- NADPH oxidase
- Poly(I:C)
- Trout
- Zymosan