Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen-Thawed without the Zona Pellucida

M. Martí; M. Grossmann; J. Santaló; J. Egozcue; M. Ponsà

doi:https://doi.org/10.1006/cryo.1996.1983

Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen-Thawed without the Zona Pellucida

M. Martí, M. Grossmann, J. Santaló, J. Egozcue, M. Ponsà

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Abstract

Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen-thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex. © 1997 Academic Press.

Original language	English
Pages (from-to)	94-106
Journal	Cryobiology
Volume	34
DOIs	https://doi.org/10.1006/cryo.1996.1983
Publication status	Published - 1 Jan 1997

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title = "Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen-Thawed without the Zona Pellucida",

abstract = "Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen-thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex. {\textcopyright} 1997 Academic Press.",

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T1 - Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen-Thawed without the Zona Pellucida

AU - Martí, M.

AU - Grossmann, M.

AU - Santaló, J.

AU - Egozcue, J.

AU - Ponsà, M.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen-thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex. © 1997 Academic Press.

AB - Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen-thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex. © 1997 Academic Press.

U2 - https://doi.org/10.1006/cryo.1996.1983

DO - https://doi.org/10.1006/cryo.1996.1983

M3 - Article

SN - 0011-2240

VL - 34

SP - 94

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JO - Cryobiology

JF - Cryobiology

ER -

Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen-Thawed without the Zona Pellucida

Abstract

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