Changes of salivary biomarkers under different storage conditions: Effects of temperature and length of storage

Tomás Barranco, Camila P. Rubio, Asta Tvarijonaviciute, Mónica Rubio, Elena Damia, Elsa Lamy, Ramón Cugat, José J. Cerón, Fernando Tecles, Damián Escribano

Research output: Contribution to journalArticleResearch

9 Citations (Scopus)

Abstract

© 2019, Biochemia Medica, Editorial Office. All rights reserved. Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2 O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at - 20 ºC and - 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2 O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2 O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At - 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2 O2. At - 80 ºC observed changes were after 3 months for TEA and H2 O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at -80 ºC than at - 20 ºC.
Original languageEnglish
Article number010706
JournalBiochemia Medica
Volume29
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • Biomarker
  • Enzyme stability
  • Oxidative stress
  • Saliva
  • Temperature

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