Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608

M. Sánchez, F. J. Gella, C. Profilis, F. Ceriotti, E. Cusó, X. Fuentes-Arderiu, K. Hallermayer, M. Hørder, M. Panteghini, G. Schumann, F. Canalias

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4 Citations (Scopus)

Abstract

We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30 ± 13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N′-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 μg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30 ± 9.65 μg/l.
Original languageEnglish
Pages (from-to)858-865
JournalClinical Chemistry and Laboratory Medicine
Volume39
DOIs
Publication statusPublished - 8 Oct 2001

Keywords

  • Creatine kinase-2
  • Reference material
  • Standardization

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