Certification of a mass concentration value to the reference material BCR 608 of creatine kinase 2

M. Sánchez Manrique, F. J. Gella Tomás, F. Canalias Reverter

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This report describes the certification of a mass concentration value to the already prepared creatine kinase 2 reference material (BCR 608) with a catalytic concentration assigned value. At present, a mass creatine kinase 2 reference method have not been described. For this reason, we measured the BCR 608 mass concentration in different immunoassays using a creatine kinase 2 purified solution as a calibrator. Previously, the protein concentration of the creatine kinase 2 solution was measured by Doestch method using the reference material SRM 927b as calibrator. Creatine kinase 2 was purified from human heart. A protein concentration value of 124.3 mg/L was assigned to the solution of purified creatine kinase 2 by Doetsch method, and then this solution was diluted to obtain a liquid standard of known protein concentration (80.362 μg/L) to calibrate the immunoassays. The mass concentration of creatine kinase 2 in the BCR 608 reference material was certified to be 93.30 μg/L with an associated uncertainty of 9.65 μg/L.
Original languageEnglish
Pages (from-to)431-436
JournalQuimica Clinica
Volume20
Issue number6
Publication statusPublished - 1 Dec 2001

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