Cerebrospinal fluid mitochondrial DNA in the Alzheimer's disease continuum

Laura Cervera-Carles, Daniel Alcolea, Ainara Estanga, Mirian Ecay-Torres, Andrea Izagirre, Montserrat Clerigué, Maite García-Sebastián, Jorge Villanúa, Clàudia Escalas, Rafael Blesa, Pablo Martínez-Lage, Alberto Lleó, Juan Fortea, Jordi Clarimón

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Abstract

© 2016 Elsevier Inc. Low levels of cell-free mitochondrial DNA (mtDNA) in the cerebrospinal fluid (CSF) of Alzheimer's disease (AD) patients have been identified and proposed as a novel biomarker for the disease. The lack of validation studies of previous results prompted us to replicate this finding in a comprehensive series of patients and controls. We applied droplet digital polymerase chain reaction in CSF specimens from 124 patients representing the AD spectrum and 140 neurologically healthy controls. The following preanalytical and analytical parameters were evaluated: the effect of freeze-thaw cycles on mtDNA, the linearity of mtDNA load across serial dilutions, and the mtDNA levels in the diagnostic groups. We found a wide range of mtDNA copies, which resulted in a high degree of overlap between groups. Although the AD group presented significantly higher mtDNA counts, the receiver-operating characteristic analysis disclosed an area under the curve of 0.715 to distinguish AD patients from controls. MtDNA was highly stable with low analytical variability. In conclusion, mtDNA levels in CSF show a high interindividual variability, with great overlap within phenotypes and presents low sensitivity for AD.
Original languageEnglish
Pages (from-to)192.e1-192.e4
JournalNeurobiology of Aging
Volume53
DOIs
Publication statusPublished - 1 May 2017

Keywords

  • Alzheimer's disease continuum
  • Cerebrospinal fluid
  • Droplet digital PCR
  • Genetic biomarkers
  • Mitochondrial DNA

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    Cervera-Carles, L., Alcolea, D., Estanga, A., Ecay-Torres, M., Izagirre, A., Clerigué, M., García-Sebastián, M., Villanúa, J., Escalas, C., Blesa, R., Martínez-Lage, P., Lleó, A., Fortea, J., & Clarimón, J. (2017). Cerebrospinal fluid mitochondrial DNA in the Alzheimer's disease continuum. Neurobiology of Aging, 53, 192.e1-192.e4. https://doi.org/10.1016/j.neurobiolaging.2016.12.009