Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia: The consistency of the process and the microbial communities involved

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Abstract

© 2017 Elsevier Ltd Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5 L and then in 50 L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50 L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3.1 ± 0.5 FPU g−1 DM and 48 ± 4 U g−1 DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2.6 · 10−3 kg volatile organic compounds per Mg of coffee husk and negligible NH3, CH4 and N2O emissions.
Original languageEnglish
Pages (from-to)1059-1068
JournalAgricultural Wastes
Volume243
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Cellulase
  • Coffee husk
  • Solid-state fermentation
  • Specialized inoculum
  • Xylanase

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