Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs

Yuan Qiu; Elena Rojas; Richard A. Murray; Joseba Irigoyen; Danijela Gregurec; Pablo Castro-Hartmann; Jana Fledderman; Irina Estrela-Lopis; Edwin Donath; Sergio E. Moya

doi:10.1039/c5nr00884k

Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs

Yuan Qiu, Elena Rojas, Richard A. Murray, Joseba Irigoyen, Danijela Gregurec, Pablo Castro-Hartmann, Jana Fledderman, Irina Estrela-Lopis, Edwin Donath, Sergio E. Moya

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

© The Royal Society of Chemistry. Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is

Original language	English
Pages (from-to)	6588-6598
Journal	Nanoscale
Volume	7
Issue number	15
DOIs	https://doi.org/10.1039/c5nr00884k
Publication status	Published - 21 Apr 2015

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Qiu, Yuan ; Rojas, Elena ; Murray, Richard A. ; Irigoyen, Joseba ; Gregurec, Danijela ; Castro-Hartmann, Pablo ; Fledderman, Jana ; Estrela-Lopis, Irina ; Donath, Edwin ; Moya, Sergio E. / Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs. In: Nanoscale. 2015 ; Vol. 7, No. 15. pp. 6588-6598.

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title = "Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs",

abstract = "{\textcopyright} The Royal Society of Chemistry. Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor{\textregistered} 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is",

author = "Yuan Qiu and Elena Rojas and Murray, {Richard A.} and Joseba Irigoyen and Danijela Gregurec and Pablo Castro-Hartmann and Jana Fledderman and Irina Estrela-Lopis and Edwin Donath and Moya, {Sergio E.}",

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Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs. / Qiu, Yuan; Rojas, Elena; Murray, Richard A.; Irigoyen, Joseba; Gregurec, Danijela; Castro-Hartmann, Pablo; Fledderman, Jana; Estrela-Lopis, Irina; Donath, Edwin; Moya, Sergio E.

In: Nanoscale, Vol. 7, No. 15, 21.04.2015, p. 6588-6598.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs

AU - Qiu, Yuan

AU - Rojas, Elena

AU - Murray, Richard A.

AU - Irigoyen, Joseba

AU - Gregurec, Danijela

AU - Castro-Hartmann, Pablo

AU - Fledderman, Jana

AU - Estrela-Lopis, Irina

AU - Donath, Edwin

AU - Moya, Sergio E.

PY - 2015/4/21

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N2 - © The Royal Society of Chemistry. Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is

AB - © The Royal Society of Chemistry. Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is

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DO - 10.1039/c5nr00884k

M3 - Article

VL - 7

SP - 6588

EP - 6598

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 15

ER -

Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs

Abstract

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