Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO<inf>2-x</inf> NPs

Yuan Qiu, Elena Rojas, Richard A. Murray, Joseba Irigoyen, Danijela Gregurec, Pablo Castro-Hartmann, Jana Fledderman, Irina Estrela-Lopis, Edwin Donath, Sergio E. Moya

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

© The Royal Society of Chemistry. Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is
Original languageEnglish
Pages (from-to)6588-6598
JournalNanoscale
Volume7
Issue number15
DOIs
Publication statusPublished - 21 Apr 2015

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