Cell lysis in Escherichia coli cultures stimulates growth and biosynthesis of recombinant proteins in surviving cells

José Luis Corchero, Rafael Cubarsí, Pau Vila, Anna Arís, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Cell growth and production of recombinant proteins in stationary phase cultures of Escherichia coli recover concomitantly with spontaneous lysis of a fraction of the ageing cell population. Further exploration of this event has indicated that sonic cell disruption stimulates both cell growth and synthesis of plasmid-encoded recombinant proteins, even in exponentially growing cultures. These observations indicate an efficient cell utilisation of released intracellular material and also that this capability is not restricted to extreme nutrient-starving conditions. In addition, the efficient re-conversion of waste cell material can be viewed as a potential strategy for an extreme exploitation of carbon sources and cell metabolites in production processes of both recombinant and non-recombinant microbial products.
Original languageEnglish
Pages (from-to)13-18
JournalMicrobiological Research
Volume156
Issue number1
DOIs
Publication statusPublished - 1 Jan 2001

Keywords

  • Ageing
  • Cell lysis
  • Growth
  • Recombinant protein
  • Stationary phase

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