Cell-free cartilage engineering approach using hyaluronic acid-polycaprolactone scaffolds: A study in vivo

M. Lebourg, S. Martínez-Díaz, N. García-Giralt, R. Torres-Claramunt, J. L.Gómez Ribelles, G. Vila-Canet, J. C. Monllau

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27 Citations (Scopus)

Abstract

Polycaprolactone scaffolds modified with cross-linked hyaluronic acid were prepared in order to establish whether a more hydrophilic and biomimetic microenvironment benefits the progenitor cells arriving from bone marrow in a cell-free tissue-engineering approach. The polycaprolactone and polycaprolactone/hyaluronic acid scaffolds were characterized in terms of morphology and water absorption capacity. The polycaprolactone and polycaprolactone/hyaluronic acid samples were implanted in a chondral defect in rabbits; bleeding of the subchondral bone was provoked to generate a spontaneous healing response. Repair at 1, 4, 12, and 24 weeks was assessed macroscopically using the International Cartilage Repair Society score and the Oswestry Arthroscopy Score and microscopically using immunohistological staining for collagen type I and type II, and for Ki-67. The presence of hyaluronic acid improves scaffold performance, which supports a good repair response without biomaterial pre-seeding. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
Original languageEnglish
Pages (from-to)1304-1315
JournalJournal of Biomaterials Applications
Volume28
Issue number9
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Cartilage regeneration
  • cell-free PCL scaffold
  • hyaluronic acid
  • osteoarthritis
  • tissue engineering

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