TY - JOUR
T1 - Ccr5 Δ32 homozygous cord blood allogeneic transplantation in a patient with hiv: A case report
AU - Duarte, Rafael F.
AU - Salgado, María
AU - Sánchez-Ortega, Isabel
AU - Arnan, Montserrat
AU - Canals, Carmen
AU - Domingo-Domenech, Eva
AU - Fernández-de-Sevilla, Alberto
AU - González-Barca, Eva
AU - Morón-López, Sara
AU - Nogues, Nuria
AU - Patiño, Beatriz
AU - Puertas, Maria Carmen
AU - Clotet, Bonaventura
AU - Petz, Lawrence D.
AU - Querol, Sergio
AU - Martinez-Picado, Javier
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Background Allogeneic donor CCR5 Δ32 homozygous haemopoietic cell transplantation (HCT) provides the only evidence to date of long-term control of HIV infection. However, availability of conventional CCR5 Δ32 homozygous donors is insuffi cient to develop this as a therapeutic strategy further. Methods We present a 37-year-old patient with HIV-1 infection and aggressive lymphoma who had disease progression after fi ve lines of radiochemotherapy including an autologous HCT, and in the absence of matched sibling donors, received an allogeneic HCT with four of six HLA-matched CCR5 Δ32 homozygous cord blood cells (StemCyte, Covina, CA), supported with purifi ed CD34+ cells from a haploidentical sibling. Blood or tissue samples were obtained before and weekly after HCT to monitor transplant and HIV infection, including chimerism analysis, CCR5 genotyping and viral tropism, viral isolation and sequence, viral reservoir analysis, immune activation and proliferation, and ex-vivo cell infectivity assays. Combined antiretroviral therapy continued during the procedure. Findings The patient's HIV was CCR5-tropic by genotypic and phenotypic analyses. Baseline latent reservoir tests showed HIV DNA copies in bulk and resting CD4 T cells and in gut-associated lymphoid tissue, CD4 T-cellassociated HIV RNA, replication competent viral size of 2·1 copies per 10 7 CD4 T cells, and single copy assay of 303 copies per mL. After HCT, plasma HIV DNA load was undetectable by ultrasensitive analyses. Upon cord blood full chimerism, the patient's CCR5 Δ32 homozygous CD4 T cells responded to proliferation and activation stimuli and became resistant to infection by the patient's viral isolate and by laboratory-adapted HIV-1 strains. Death related to lymphoma progression regretfully prevented long-term monitoring of the patient's viral reservoir. Interpretation CCR5 Δ32 homozygous cord blood reconstitution can successfully eliminate HIV-1 and render the allogeneic graft recipient's T lymphocytes resistant to HIV infection. Thus, they build on the evidence available to strongly support the use of cord blood as a strategic platform for a broader application of non-functional CCR5 transplantation to other infected individuals. Funding Spanish Secretariat of Research, the American Foundation for AIDS Research (amfAR).
AB - Background Allogeneic donor CCR5 Δ32 homozygous haemopoietic cell transplantation (HCT) provides the only evidence to date of long-term control of HIV infection. However, availability of conventional CCR5 Δ32 homozygous donors is insuffi cient to develop this as a therapeutic strategy further. Methods We present a 37-year-old patient with HIV-1 infection and aggressive lymphoma who had disease progression after fi ve lines of radiochemotherapy including an autologous HCT, and in the absence of matched sibling donors, received an allogeneic HCT with four of six HLA-matched CCR5 Δ32 homozygous cord blood cells (StemCyte, Covina, CA), supported with purifi ed CD34+ cells from a haploidentical sibling. Blood or tissue samples were obtained before and weekly after HCT to monitor transplant and HIV infection, including chimerism analysis, CCR5 genotyping and viral tropism, viral isolation and sequence, viral reservoir analysis, immune activation and proliferation, and ex-vivo cell infectivity assays. Combined antiretroviral therapy continued during the procedure. Findings The patient's HIV was CCR5-tropic by genotypic and phenotypic analyses. Baseline latent reservoir tests showed HIV DNA copies in bulk and resting CD4 T cells and in gut-associated lymphoid tissue, CD4 T-cellassociated HIV RNA, replication competent viral size of 2·1 copies per 10 7 CD4 T cells, and single copy assay of 303 copies per mL. After HCT, plasma HIV DNA load was undetectable by ultrasensitive analyses. Upon cord blood full chimerism, the patient's CCR5 Δ32 homozygous CD4 T cells responded to proliferation and activation stimuli and became resistant to infection by the patient's viral isolate and by laboratory-adapted HIV-1 strains. Death related to lymphoma progression regretfully prevented long-term monitoring of the patient's viral reservoir. Interpretation CCR5 Δ32 homozygous cord blood reconstitution can successfully eliminate HIV-1 and render the allogeneic graft recipient's T lymphocytes resistant to HIV infection. Thus, they build on the evidence available to strongly support the use of cord blood as a strategic platform for a broader application of non-functional CCR5 transplantation to other infected individuals. Funding Spanish Secretariat of Research, the American Foundation for AIDS Research (amfAR).
U2 - 10.1016/S2352-3018(15)00083-1
DO - 10.1016/S2352-3018(15)00083-1
M3 - Article
VL - 2
SP - e236-e242
JO - The Lancet HIV
JF - The Lancet HIV
SN - 2352-3018
IS - 6
M1 - e228
ER -
