Cas9 in Human Embryos: On Target but No Repair

Eva R. Hoffmann; Ignasi Roig

doi:10.1016/j.cell.2020.11.022

Cas9 in Human Embryos: On Target but No Repair

Eva R. Hoffmann^*, Ignasi Roig

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for “gene surgery”.

Original language	English
Pages (from-to)	1464-1466
Number of pages	3
Journal	Cell
Volume	183
Issue number	6
DOIs	https://doi.org/10.1016/j.cell.2020.11.022
Publication status	Published - 10 Dec 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.cell.2020.11.022

Cite this

@article{6db0d1e04c8b492f8c48709372364c76,

title = "Cas9 in Human Embryos: On Target but No Repair",

abstract = "In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for “gene surgery”.",

author = "Hoffmann, {Eva R.} and Ignasi Roig",

note = "Funding Information: E.R.H. is funded by an ERC ( 724718-ReCAP ), Novo Nordisk Foundation ( NNF15OC0016662 ), and a Danish National Research Foundation Center Grant ( 6110-00344B ). I.R. is supported by the Spanish Ministerio de Ciencia e Innovaci{\'o}n ( PID2019-107082RB-I00 ) and Fundaci{\'o}n Dexeus Mujer ( DEXEUS2019-001 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = dec,

day = "10",

doi = "10.1016/j.cell.2020.11.022",

language = "English",

volume = "183",

pages = "1464--1466",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

}

TY - JOUR

T1 - Cas9 in Human Embryos

T2 - On Target but No Repair

AU - Hoffmann, Eva R.

AU - Roig, Ignasi

N1 - Funding Information: E.R.H. is funded by an ERC ( 724718-ReCAP ), Novo Nordisk Foundation ( NNF15OC0016662 ), and a Danish National Research Foundation Center Grant ( 6110-00344B ). I.R. is supported by the Spanish Ministerio de Ciencia e Innovación ( PID2019-107082RB-I00 ) and Fundación Dexeus Mujer ( DEXEUS2019-001 ). Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/12/10

Y1 - 2020/12/10

N2 - In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for “gene surgery”.

AB - In this issue of Cell, Zuccaro and colleagues show that on-target Cas9-mediated double-strand breaks cause chromosome loss or mis-repair of the disease allele in > 90% of human embryos. End joining repair pathways dominate, causing small insertions or deletions, which raises serious questions about using double-strand breaks for “gene surgery”.

UR - http://www.scopus.com/inward/record.url?scp=85097462464&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2020.11.022

DO - 10.1016/j.cell.2020.11.022

M3 - Article

C2 - 33306952

AN - SCOPUS:85097462464

SN - 0092-8674

VL - 183

SP - 1464

EP - 1466

JO - Cell

JF - Cell

IS - 6

ER -

Cas9 in Human Embryos: On Target but No Repair

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this