Carbon nanotube composite as novel platform for microbial biosensor

Ülkü A. Kirgoz, Suna Timur, Dilek Odaci, Briza Pérez, Salvador Alegret, Arben Merkoçi

Research output: Contribution to journalArticleResearchpeer-review

37 Citations (Scopus)

Abstract

The presented work includes the development of a microbial biosensor based on a carbon-nanotube epoxy composite (CNTEC) platform used as supporting electrode for cell immobilization. For this purpose, cells of Pseudomonas fluorescens were immobilized on the surface of the CNTEC electrode by means of gelatin which was then cross linked with glutaraldehyde. After optimization of experimental parameters like cell amount, pH and temperature, the system was calibrated for glucose. From the calibration graph the linear range was estimated as 0.5-4.0 mM with a response time of 100 s. Furthermore, substrate specificity and operational stability were investigated. Finally, the results that were obtained with CNTEC electrodes were compared with conventional graphite epoxy composite electrode (GECE) and as a result, higher current values (2 to 3 folds) were observed with CNTEC microbial biosensor. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Original languageEnglish
Pages (from-to)893-898
JournalElectroanalysis
Volume19
Issue number7-8
DOIs
Publication statusPublished - 1 Apr 2007

Keywords

  • Carbon nanotube
  • Graphite-epoxy composite
  • Microbial biosensor
  • Pseudomonas fluorescens

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