Carbohydrate hydrolytic potential and redundancy of an anaerobic digestion microbiome exposed to acidosis, as uncovered by metagenomics

Marie Bertucci, Magdalena Calusinska, Xavier Goux, Corinne Rouland-Lefèvre, Boris Untereiner, Pau Ferrer, Patrick A. Gerin, Philippe Delfosse

Research output: Contribution to journalArticleResearch

6 Citations (Scopus)

Abstract

© 2019 American Society for Microbiology. Increased hydrolysis of easily digestible biomass may lead to acidosis of anaerobic reactors and decreased methane production. Previously, it was shown that the structure of microbial communities changed during acidosis; however, once the conditions are back to optimal, biogas (initially CO2) production quickly restarts. This suggests the retention of the community functional redundancy during the process failure. In this study, with the use of metagenomics and downstream bioinformatics analyses, we characterize the carbohydrate hydrolytic potential of the microbial community, with a special focus on acidosis. To that purpose, carbohydrate-active enzymes were identified, and to further link the community hydrolytic potential with key microbes, bacterial genomes were reconstructed. In addition, we characterized biochemically the specificity and activity of selected enzymes, thus verifying the accuracy of the in silico predictions. The results confirm the retention of the community hydrolytic potential during acidosis and indicate Bacteroidetes to be largely involved in biomass degradation. Bacteroidetes showed higher diversity and genomic content of carbohydrate hydrolytic enzymes that might favor the dominance of this phylum over other bacteria in some anaerobic reactors. The combination of bioinformatic analyses and activity tests enabled us to propose a model of acetylated glucomannan degradation by Bacteroidetes.
Original languageEnglish
Article numberUNSP e00895-19
Number of pages16
JournalApplied and Environmental Microbiology
Volume85
Issue number15
DOIs
Publication statusPublished - 1 Aug 2019

Keywords

  • BACTEROIDES-THETAIOTAOMICRON
  • Bacteroidetes
  • CELLOBIOSE 2-EPIMERASE
  • CELLULOSE DEGRADATION
  • COMMUNITY STRUCTURE
  • FUNCTIONAL-CHARACTERIZATION
  • GUT
  • KONJAC GLUCOMANNAN
  • MACROTERMES-MULLERI
  • MANNAN CATABOLIC PATHWAY
  • POLYSACCHARIDE
  • biotechnology
  • enzymes
  • molecular biology
  • polysaccharides
  • recombinant-protein production

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