Capillary gas chromatography-mass spectrometry quantitative determination of hydroxytyrosol and tyrosol in human urine after olive oil intake

Elisabet Miró-Casas, Magí Farré Albaladejo, Maria Isabel Covas, Jordi Ortuño Rodriguez, Ester Menoyo Colomer, Rosa M. Lamuela Raventós, Rafael De La Torre

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Abstract

Recent in vitro studies have demonstrated antioxidant properties of some virgin olive oil phenolic compounds. One of the prerequisites to extrapolate these data to an in vivo situation is the knowledge of their bioavailability in humans. In the present work we describe an analytical method which enables us to perform hydroxytyrosol and tyrosol quantitative determinations in human urine. This method was successfully used in bioavailability studies of both phenolic compounds after acute olive oil administration. Virgin olive oil was administered to healthy volunteers after a low phenolic diet. The dose administered of both phenolic compounds was estimated in reference to free forms of hydroxytyrosol and tyrosol present in virgin olive oil extracts before and after being submitted to hydrolytic conditions. These conditions mimic those occurring during digestion. Urine samples were collected before and after acute olive oil intake and analyzed by capillary gas chromatography-mass spectrometry. Hydroxytyrosol and tyrosol urinary recovery increased in response to olive oil administration, obtaining maximal values in the first 4 h. Our results further indicate that hydroxytyrosol and tyrosol are mainly excreted in conjugated form, since only 5.9 ± 1.4% (hydroxytyrosol) and 13.8 ± 5.4% (tyrosol) of the total amounts excreted in urine were in free form. © 2001 Academic Press.
Original languageEnglish
Pages (from-to)63-72
JournalAnalytical Biochemistry
Volume294
DOIs
Publication statusPublished - 1 Jul 2001

Keywords

  • Bioavailability in human
  • Gas chromatography
  • Hydroxytyrosol
  • Mass spectrometry
  • Olive oil phenolic compounds
  • Tyrosol

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    Miró-Casas, E., Farré Albaladejo, M., Covas, M. I., Rodriguez, J. O., Menoyo Colomer, E., Lamuela Raventós, R. M., & De La Torre, R. (2001). Capillary gas chromatography-mass spectrometry quantitative determination of hydroxytyrosol and tyrosol in human urine after olive oil intake. Analytical Biochemistry, 294, 63-72. https://doi.org/10.1006/abio.2001.5160