Canine Mhc DRB1 genotyping by PCR-RFLP analysis

O. Francino; M. Amills; A. Sánchez

doi:https://doi.org/10.1111/j.1365-2052.1997.00062.x

Canine Mhc DRB1 genotyping by PCR-RFLP analysis

O. Francino, M. Amills, A. Sánchez

Research output: Contribution to journal › Article › Research › peer-review

26 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) genotyping procedure has been developed for the canine major histocompatibility complex DRB1 gene (Cafo-DRB1), which allows us to distinguish all the DRB1 alleles described to date and reveals the existence of new ones. The polymorphic second exon of the Cafa-DRB1 gene was amplified and the product analysed for restriction fragment length polymorphism (RFLP) with the enzymes RsaI, MboI, TaqI and AspHI. Nine RFLP combinations could be associated with previously known alleles. Two new RFLP combinations corresponded to new alleles and were confirmed by DNA sequencing (Cafa-DRB1*10 and Cafa-DRB1*11). Close associations between RFLPs in the DRB1 second exon and the presence of specific amine acid residues included in the side-chain pockets of the antigen-binding site of the DR molecule are also described.

Original language	English
Pages (from-to)	41-45
Journal	Animal Genetics
Volume	28
DOIs	https://doi.org/10.1111/j.1365-2052.1997.00062.x
Publication status	Published - 1 Jan 1997

Keywords

DRB
MHC class II genes
PCR-RFLP
antigen binding site
dog
polymorphism

Access to Document

https://doi.org/10.1111/j.1365-2052.1997.00062.x

Cite this

@article{3ca2e4fdf40846bbb5a81d5b19c1ff88,

title = "Canine Mhc DRB1 genotyping by PCR-RFLP analysis",

abstract = "A polymerase chain reaction (PCR) genotyping procedure has been developed for the canine major histocompatibility complex DRB1 gene (Cafo-DRB1), which allows us to distinguish all the DRB1 alleles described to date and reveals the existence of new ones. The polymorphic second exon of the Cafa-DRB1 gene was amplified and the product analysed for restriction fragment length polymorphism (RFLP) with the enzymes RsaI, MboI, TaqI and AspHI. Nine RFLP combinations could be associated with previously known alleles. Two new RFLP combinations corresponded to new alleles and were confirmed by DNA sequencing (Cafa-DRB1*10 and Cafa-DRB1*11). Close associations between RFLPs in the DRB1 second exon and the presence of specific amine acid residues included in the side-chain pockets of the antigen-binding site of the DR molecule are also described.",

keywords = "DRB, MHC class II genes, PCR-RFLP, antigen binding site, dog, polymorphism",

author = "O. Francino and M. Amills and A. S{\'a}nchez",

year = "1997",

month = jan,

day = "1",

doi = "https://doi.org/10.1111/j.1365-2052.1997.00062.x",

language = "English",

volume = "28",

pages = "41--45",

journal = "Animal Genetics",

issn = "0268-9146",

}

TY - JOUR

T1 - Canine Mhc DRB1 genotyping by PCR-RFLP analysis

AU - Francino, O.

AU - Amills, M.

AU - Sánchez, A.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - A polymerase chain reaction (PCR) genotyping procedure has been developed for the canine major histocompatibility complex DRB1 gene (Cafo-DRB1), which allows us to distinguish all the DRB1 alleles described to date and reveals the existence of new ones. The polymorphic second exon of the Cafa-DRB1 gene was amplified and the product analysed for restriction fragment length polymorphism (RFLP) with the enzymes RsaI, MboI, TaqI and AspHI. Nine RFLP combinations could be associated with previously known alleles. Two new RFLP combinations corresponded to new alleles and were confirmed by DNA sequencing (Cafa-DRB1*10 and Cafa-DRB1*11). Close associations between RFLPs in the DRB1 second exon and the presence of specific amine acid residues included in the side-chain pockets of the antigen-binding site of the DR molecule are also described.

AB - A polymerase chain reaction (PCR) genotyping procedure has been developed for the canine major histocompatibility complex DRB1 gene (Cafo-DRB1), which allows us to distinguish all the DRB1 alleles described to date and reveals the existence of new ones. The polymorphic second exon of the Cafa-DRB1 gene was amplified and the product analysed for restriction fragment length polymorphism (RFLP) with the enzymes RsaI, MboI, TaqI and AspHI. Nine RFLP combinations could be associated with previously known alleles. Two new RFLP combinations corresponded to new alleles and were confirmed by DNA sequencing (Cafa-DRB1*10 and Cafa-DRB1*11). Close associations between RFLPs in the DRB1 second exon and the presence of specific amine acid residues included in the side-chain pockets of the antigen-binding site of the DR molecule are also described.

KW - DRB

KW - MHC class II genes

KW - PCR-RFLP

KW - antigen binding site

KW - dog

KW - polymorphism

U2 - https://doi.org/10.1111/j.1365-2052.1997.00062.x

DO - https://doi.org/10.1111/j.1365-2052.1997.00062.x

M3 - Article

SN - 0268-9146

VL - 28

SP - 41

EP - 45

JO - Animal Genetics

JF - Animal Genetics

ER -

Canine Mhc DRB1 genotyping by PCR-RFLP analysis

Abstract

Keywords

Access to Document

Fingerprint

Cite this