TY - JOUR
T1 - Can we rule out a congenital cytomegalovirus infection when the result of polymerase chain reaction in dried blood spots is negative?
AU - Vives-Oñós, Isabel
AU - Soler-Palacín, Pere
AU - Codina-Grau, María Gemma
AU - Martín-Nalda, Andrea
AU - López-Galera, Rosa María
AU - Marín-Soria, José Luís
AU - Figueras-Nadal, Concepció
PY - 2014/1/1
Y1 - 2014/1/1
N2 - © 2013 Elsevier España, S.L.U. All rights reserved. Introduction: The detection of cytomegalovirus (CMV) DNA by real time polymerase chain reaction (rt-PCR) in dried blood spots collected routinely for metabolic screening has been assessed for the retrospective diagnosis of congenital CMV (cCMV) infection in many studies, but not in Spain. The aim of this study is to analyze the diagnostic accuracy of this technique in our hospital. Methods: A cross-sectional retrospective observational study was conducted including all patients born between January, 2007 and September, 2012 with confirmed cCMV infection. The assessment of CMV DNA was made by using rt-PCR in dried blood spots of these patients. Results: Fourteen patients were included: 4/14 were symptomatic and 4/14 had sequelae. The detection of CMV DNA by rt-PCR was positive in only 7 patients. A statistically significant relationship between low viral load at birth and negative rt-PCR in dried blood spots was demonstrated. Conclusions: Despite the low number of patients included, our data highlight an important amount of false negative results in the DNA CMV detection by rt-PCR in these samples for the retrospective diagnosis of cCMV infection, especially in cases with low viral load at birth.
AB - © 2013 Elsevier España, S.L.U. All rights reserved. Introduction: The detection of cytomegalovirus (CMV) DNA by real time polymerase chain reaction (rt-PCR) in dried blood spots collected routinely for metabolic screening has been assessed for the retrospective diagnosis of congenital CMV (cCMV) infection in many studies, but not in Spain. The aim of this study is to analyze the diagnostic accuracy of this technique in our hospital. Methods: A cross-sectional retrospective observational study was conducted including all patients born between January, 2007 and September, 2012 with confirmed cCMV infection. The assessment of CMV DNA was made by using rt-PCR in dried blood spots of these patients. Results: Fourteen patients were included: 4/14 were symptomatic and 4/14 had sequelae. The detection of CMV DNA by rt-PCR was positive in only 7 patients. A statistically significant relationship between low viral load at birth and negative rt-PCR in dried blood spots was demonstrated. Conclusions: Despite the low number of patients included, our data highlight an important amount of false negative results in the DNA CMV detection by rt-PCR in these samples for the retrospective diagnosis of cCMV infection, especially in cases with low viral load at birth.
KW - Congenital, hereditary, and neonatal
KW - Cytomegalovirus infection
KW - Diseases and abnormalities
KW - Dried blood spot testing
KW - Neonatal screening
KW - Real-time polymerase chain reaction
KW - Viral DNA
U2 - 10.1016/j.eimc.2013.09.018
DO - 10.1016/j.eimc.2013.09.018
M3 - Article
VL - 32
SP - 570
EP - 573
JO - Enfermedades Infecciosas y Microbiologia Clinica
JF - Enfermedades Infecciosas y Microbiologia Clinica
SN - 0213-005X
IS - 9
ER -