Calorimetry of microbial growth using a thermopile based microreactor

J. Higuera-Guisset, J. Rodríguez-Viejo, M. Chacón, F. J. Muñoz, N. Vigués, J. Mas

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41 Citations (Scopus)

Abstract

A miniaturized calorimeter, based on silicon integrated thermopile chips, has been developed for the determination of growth-related heat production in microbial cultures. The calorimetric vessels consists of two independent sensors located within a thermostated aluminum frame, the heat sink, and covered by a 0.6 ml reaction chamber made of PTFE for improved thermal insulation. The second sensor was used as a reference to minimize temperature perturbations on the output signal. Baseline stability was better than 0.08 μW h-1. The Si thin-film membrane which supports the Al-Si thermopiles enabled an excellent dynamic response and a temperature resolution of 50 μK. The sensitivity for the heat power measurement was 0.39 V W-1. Batch measurements of Escherichia coli activity under different conditions have been performed. The thermal profiles matched the exponential growth kinetics usually found in batch cultures of bacteria. A simplified model based in the Monod equation is used to analyze the influence of oxygen depletion on cell growth. © 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)187-191
JournalThermochimica Acta
Volume427
DOIs
Publication statusPublished - 1 Jan 2005

Keywords

  • Bacterial growth
  • Heat-flow microcalorimeter
  • Metabolic activity
  • Thermopile silicon chip

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