Calcium-mobilizing effectors inhibit P-enolpyruvate carboxykinase gene expression in cultured rat hepatocytes

Alfons Valera; Gemma Solanes; Fatima Bosch

doi:10.1016/0014-5793(93)80679-O

Calcium-mobilizing effectors inhibit P-enolpyruvate carboxykinase gene expression in cultured rat hepatocytes

Alfons Valera, Gemma Solanes, Fatima Bosch

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Incubation of primary cultures of hepatocytes from fed and fasted rats with calcium ionophore strongly decreased glucose production from pyruvate. Like insulin, calcium ionophore A23187, phenylephrine, vasopressin, and prostaglandins E2 and F2α caused a significant reduction (50-60%) in basal concentrations of mRNA for P-enolpyruvate carboxykinase (PEPCK), the main regulatory enzyme of gluconeogenesis. Phenylephrine, prostaglandin E2 and calcium ionophore A23187 were also able to counteract the induction of PEPCK gene expression by Bt2cAMP. These effects were similar to those exerted by both vanadate and phorbol ester TPA. The decrease in extracellular calcium by the addition of the calcium-chelating agent EGTA to the incubation medium caused an increase in PEPCK mRNA levels. This effect was additive to that of Bt2cAMP and was counteracted by vanadate. © 1993.

Original language	English
Pages (from-to)	319-324
Journal	FEBS Letters
Volume	333
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80679-O
Publication status	Published - 1 Nov 1993

Keywords

Calcium ionophore A23187
Gene expression
Insulin
P-enolpyruvate carboxykinase
Phenylephrine

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10.1016/0014-5793(93)80679-O

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title = "Calcium-mobilizing effectors inhibit P-enolpyruvate carboxykinase gene expression in cultured rat hepatocytes",

abstract = "Incubation of primary cultures of hepatocytes from fed and fasted rats with calcium ionophore strongly decreased glucose production from pyruvate. Like insulin, calcium ionophore A23187, phenylephrine, vasopressin, and prostaglandins E2 and F2α caused a significant reduction (50-60%) in basal concentrations of mRNA for P-enolpyruvate carboxykinase (PEPCK), the main regulatory enzyme of gluconeogenesis. Phenylephrine, prostaglandin E2 and calcium ionophore A23187 were also able to counteract the induction of PEPCK gene expression by Bt2cAMP. These effects were similar to those exerted by both vanadate and phorbol ester TPA. The decrease in extracellular calcium by the addition of the calcium-chelating agent EGTA to the incubation medium caused an increase in PEPCK mRNA levels. This effect was additive to that of Bt2cAMP and was counteracted by vanadate. {\textcopyright} 1993.",

keywords = "Calcium ionophore A23187, Gene expression, Insulin, P-enolpyruvate carboxykinase, Phenylephrine",

author = "Alfons Valera and Gemma Solanes and Fatima Bosch",

year = "1993",

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doi = "10.1016/0014-5793(93)80679-O",

language = "English",

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T1 - Calcium-mobilizing effectors inhibit P-enolpyruvate carboxykinase gene expression in cultured rat hepatocytes

AU - Valera, Alfons

AU - Solanes, Gemma

AU - Bosch, Fatima

PY - 1993/11/1

Y1 - 1993/11/1

N2 - Incubation of primary cultures of hepatocytes from fed and fasted rats with calcium ionophore strongly decreased glucose production from pyruvate. Like insulin, calcium ionophore A23187, phenylephrine, vasopressin, and prostaglandins E2 and F2α caused a significant reduction (50-60%) in basal concentrations of mRNA for P-enolpyruvate carboxykinase (PEPCK), the main regulatory enzyme of gluconeogenesis. Phenylephrine, prostaglandin E2 and calcium ionophore A23187 were also able to counteract the induction of PEPCK gene expression by Bt2cAMP. These effects were similar to those exerted by both vanadate and phorbol ester TPA. The decrease in extracellular calcium by the addition of the calcium-chelating agent EGTA to the incubation medium caused an increase in PEPCK mRNA levels. This effect was additive to that of Bt2cAMP and was counteracted by vanadate. © 1993.

AB - Incubation of primary cultures of hepatocytes from fed and fasted rats with calcium ionophore strongly decreased glucose production from pyruvate. Like insulin, calcium ionophore A23187, phenylephrine, vasopressin, and prostaglandins E2 and F2α caused a significant reduction (50-60%) in basal concentrations of mRNA for P-enolpyruvate carboxykinase (PEPCK), the main regulatory enzyme of gluconeogenesis. Phenylephrine, prostaglandin E2 and calcium ionophore A23187 were also able to counteract the induction of PEPCK gene expression by Bt2cAMP. These effects were similar to those exerted by both vanadate and phorbol ester TPA. The decrease in extracellular calcium by the addition of the calcium-chelating agent EGTA to the incubation medium caused an increase in PEPCK mRNA levels. This effect was additive to that of Bt2cAMP and was counteracted by vanadate. © 1993.

KW - Calcium ionophore A23187

KW - Gene expression

KW - Insulin

KW - P-enolpyruvate carboxykinase

KW - Phenylephrine

U2 - 10.1016/0014-5793(93)80679-O

DO - 10.1016/0014-5793(93)80679-O

M3 - Article

VL - 333

SP - 319

EP - 324

IS - 3

ER -

Calcium-mobilizing effectors inhibit P-enolpyruvate carboxykinase gene expression in cultured rat hepatocytes

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Keywords

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