Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain

A. Toledo, R. Rodriguez, J. Sabriá, J. Rodriguez, I. Blanco

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright © 1991, Wiley Blackwell. All rights reserved
Original languageEnglish
Pages (from-to)380-384
JournalJournal of Neurochemistry
Volume56
DOIs
Publication statusPublished - 1 Jan 1991

Keywords

  • Brain
  • Calcium
  • Histidine decarboxylase
  • Membrane‐bound enzyme

Fingerprint

Dive into the research topics of 'Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain'. Together they form a unique fingerprint.

Cite this