Abstract
Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright © 1991, Wiley Blackwell. All rights reserved
Original language | English |
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Pages (from-to) | 380-384 |
Journal | Journal of Neurochemistry |
Volume | 56 |
DOIs | |
Publication status | Published - 1 Jan 1991 |
Keywords
- Brain
- Calcium
- Histidine decarboxylase
- Membrane‐bound enzyme