Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain

A. Toledo; R. Rodriguez; J. Sabriá; J. Rodriguez; I. Blanco

doi:https://doi.org/10.1111/j.1471-4159.1991.tb08162.x

Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain

A. Toledo, R. Rodriguez, J. Sabriá, J. Rodriguez, I. Blanco

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright © 1991, Wiley Blackwell. All rights reserved

Original language	English
Pages (from-to)	380-384
Journal	Journal of Neurochemistry
Volume	56
DOIs	https://doi.org/10.1111/j.1471-4159.1991.tb08162.x
Publication status	Published - 1 Jan 1991

Keywords

Brain
Calcium
Histidine decarboxylase
Membrane‐bound enzyme

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https://doi.org/10.1111/j.1471-4159.1991.tb08162.x

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title = "Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain",

abstract = "Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright {\textcopyright} 1991, Wiley Blackwell. All rights reserved",

keywords = "Brain, Calcium, Histidine decarboxylase, Membrane‐bound enzyme",

author = "A. Toledo and R. Rodriguez and J. Sabri{\'a} and J. Rodriguez and I. Blanco",

year = "1991",

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doi = "https://doi.org/10.1111/j.1471-4159.1991.tb08162.x",

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T1 - Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain

AU - Toledo, A.

AU - Rodriguez, R.

AU - Sabriá, J.

AU - Rodriguez, J.

AU - Blanco, I.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright © 1991, Wiley Blackwell. All rights reserved

AB - Abstract: In a previous work we have shown that histidine decarboxylase (HD) activity is found in a soluble and a membrane‐bound form. A major part (82%) of the membrane‐bound HD activity in the crude mitochondrial fraction (P2) was present in the synaptic plasma membrane‐containing subfraction. Physiological concentrations of Ca2+ had no direct effect on HD activity but caused a solubilization of ∼50% of membrane‐bound HD in the P2 fraction. Mg2+ had similar but lower effects (20% solubilization) than Ca2+. Incubation with depolarizing concentrations of K+ in the presence of 1 mM CaCl2 caused a significant (30%) solubilization of HD. Copyright © 1991, Wiley Blackwell. All rights reserved

KW - Brain

KW - Calcium

KW - Histidine decarboxylase

KW - Membrane‐bound enzyme

U2 - https://doi.org/10.1111/j.1471-4159.1991.tb08162.x

DO - https://doi.org/10.1111/j.1471-4159.1991.tb08162.x

M3 - Article

VL - 56

SP - 380

EP - 384

ER -

Calcium Effects on the Solubilization of Membrane‐Bound Histidine Decarboxylase in the Rat Brain

Abstract

Keywords

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