Calcium dobesilate prevents the oxidative stress and inflammation induced by diabetes in the retina of db/db mice

Patricia Bogdanov, Cristina Solà-Adell, Cristina Hernández, Marta García-Ramírez, Joel Sampedro, Olga Simó-Servat, Marta Valeri, Christian Pasquali, Rafael Simó

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

© 2017 Elsevier Inc. Aim Calcium dobesilate (CaD) is beneficial in early stages of diabetic retinopathy (DR), but its mechanisms of action remains to be elucidated. The aim was to investigate the effect of CaD on proinflammatory cytokines and oxidative stress. Methods db/db mice were randomly assigned to daily oral treatment with CaD (200 mg/kg/day) or vehicle for 15 days. Biomarkers of oxidative stress (dihydroethidium, malondialdehyde), NF-κB, and proinflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α, MCP-1) were examined in the retina by immunohistochemical analysis. Cultures of human retinal endothelial cells (HRECs) were used for complementary experiments. Results CaD significantly reduced the biomarkers of oxidative stress in the retina of db/db mice. In addition, CaD prevented the increase of NF-κB, IL-6, IL-8, TNF-α and MCP-1 induced by diabetes. CaD inhibited the activation of NF-kβ induced by IL-1β by preventing IKKB-α phosphorylation in HRECs and reduced the upregulation of IL-6 and IL-18 induced by TNF-α in a dose-dependent manner. Conclusion Our results suggest that antioxidant and antiinflammatory effects are crucial in accounting for the effectiveness of CaD for treating DR.
Original languageEnglish
Pages (from-to)1481-1490
JournalJournal of Diabetes and its Complications
Volume31
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Calcium dobesilate
  • Diabetic retinopathy
  • Inflammation
  • Oxidative stress
  • Retinal endothelial cells
  • db/db mouse

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