Calcium- and potassium-permeable plasma membrane transporters are activated by copper in Arabidopsis root tips: Linking copper transport with cytosolic hydroxyl radical production

Ana Rodrigo-Moreno, Nuria Andrés-Colás, Charlotte Poschenrieder, Benet Gunsé, Lola Peñarrubia, Sergey Shabala

Research output: Contribution to journalArticleResearchpeer-review

53 Citations (Scopus)

Abstract

Transition metals such as copper can interact with ascorbate or hydrogen peroxide to form highly reactive hydroxyl radicals (OH•), with numerous implications to membrane transport activity and cell metabolism. So far, such interaction was described for extracellular (apoplastic) space but not cytosol. Here, a range of advanced electrophysiological and imaging techniques were applied to Arabidopsis thaliana plants differing in their copper-transport activity: Col-0, high-affinity copper transporter COPT1-overexpressing (C1OE) seedlings, and T-DNA COPT1 insertion mutant (copt1). Low Cu concentrations (10μm) stimulated a dose-dependent Gd3+ and verapamil sensitive net Ca2+ influx in the root apex but not in mature zone. C1OE also showed a fivefold higher Cu-induced K+ efflux at the root tip level compared with Col-0, and a reduction in basal peroxide accumulation at the root tip after copper exposure. Copper caused membrane disruptions of the root apex in C1OE seedlings but not in copt1 plants; this damage was prevented by pretreatment with Gd3+. Our results suggest that copper transport into cytosol in root apex results in hydroxyl radical generation at the cytosolic side, with a consequent regulation of plasma membrane OH•-sensitive Ca2+ and K+ transport systems. © 2012 Blackwell Publishing Ltd.
Original languageEnglish
Pages (from-to)844-855
JournalPlant, Cell and Environment
Volume36
Issue number4
DOIs
Publication statusPublished - 1 Apr 2013

Keywords

  • Arabidopsis thaliana
  • Ca 2+
  • COPT1
  • K +
  • Oxidative stress
  • Toxicity

Fingerprint Dive into the research topics of 'Calcium- and potassium-permeable plasma membrane transporters are activated by copper in Arabidopsis root tips: Linking copper transport with cytosolic hydroxyl radical production'. Together they form a unique fingerprint.

  • Cite this