TY - CHAP
T1 - Building Up Functional Coiled-Coil-Based Supramolecular Assemblies for Biomedical and Biotechnological Applications
AU - Fornt-Suñé, Marc
AU - Garcia-Pardo, Javier
AU - Ventura, Salvador
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2024/11/14
Y1 - 2024/11/14
N2 - The self-assembling nature of coiled-coils has brought this common structural motif into the spotlight of protein design since it offers a customizable framework for engineering innovative protein nanoparticles with tailored functionalities. We recently harnessed the self-assembling capabilities of ZapB, a bacterial coiled-coil protein, to build up fluorescent protein nanoparticles possessing remarkable affinity for antibodies. Here, we describe a complete workflow detailing the design, production, and characterization of such coiled-coil-based protein nanostructures. Additionally, we detail their functionalization with specific antibodies and illustrate their utility in stimulating the activation and proliferation of human T cells, underscoring the potential of these protein-only nanoparticles in immunotherapeutic interventions.
AB - The self-assembling nature of coiled-coils has brought this common structural motif into the spotlight of protein design since it offers a customizable framework for engineering innovative protein nanoparticles with tailored functionalities. We recently harnessed the self-assembling capabilities of ZapB, a bacterial coiled-coil protein, to build up fluorescent protein nanoparticles possessing remarkable affinity for antibodies. Here, we describe a complete workflow detailing the design, production, and characterization of such coiled-coil-based protein nanostructures. Additionally, we detail their functionalization with specific antibodies and illustrate their utility in stimulating the activation and proliferation of human T cells, underscoring the potential of these protein-only nanoparticles in immunotherapeutic interventions.
KW - Antibody
KW - Coiled-coil
KW - Protein nanoparticles
KW - Self-assembly
KW - Supramolecular assemblies
KW - T-cell activation
KW - T-cell proliferation
KW - Z-domain
KW - Nanostructures/chemistry
KW - T-Lymphocytes/immunology
KW - Humans
KW - Biotechnology/methods
KW - Protein Engineering/methods
KW - Nanoparticles/chemistry
KW - Bacterial Proteins/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85209828641&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/be85c36c-9897-3b24-83e3-529f29b11dbe/
U2 - 10.1007/978-1-0716-4213-9_13
DO - 10.1007/978-1-0716-4213-9_13
M3 - Chapter
C2 - 39543039
AN - SCOPUS:85209828641
SN - 978-1-0716-4212-2
VL - 2870
T3 - Methods in Molecular Biology
SP - 245
EP - 265
BT - Methods in Molecular Biology
ER -