© 2017 Future Medicine Ltd. Aim: The bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity. Patients & methods: Patient sputum samples from 21 patients were studied. Results: Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances <1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances >50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven. Conclusion: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.
- 16S rRNA
- lung microbiome
- P. aeruginosa
- Severe COPD
Garcia-Nuñez, M., Marti, S., Puig, C., Perez-Brocal, V., Millares, L., Santos, S., Ardanuy, C., Moya, A., Linãres, J., & Monsó, E. (2017). Bronchial microbiome, PA biofilm-forming capacity and exacerbation in severe COPD patients colonized by P. aeruginosa. Future Microbiology, 12(5), 379-392. https://doi.org/10.2217/fmb-2016-0127