TY - JOUR
T1 - Broadband homodecoupled time-shared 1H-13C and 1H-15N HSQC experiments
AU - Nolis, Pau
AU - Motiram-Corral, Kumar
AU - Pérez-Trujillo, Míriam
AU - Parella, Teodor
N1 - Funding Information:
Financial support for this research provided by Spanish MINECO (project CTQ2015-64436-P ) is gratefully acknowledged. We also thank the Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, for allocating instrument time to this project.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/1
Y1 - 2019/1
N2 - The concepts of pure-shift NMR and time-shared NMR are merged in a single experiment. A 13C/15N time-shared version of the real-time BIRD-based broadband homodecoupled HSQC experiment is described. This time-efficient approach affords simultaneously 1H-13C and 1H-15N pure-shift HSQC spectra in a single acquisition, while achieving substantial gains in both sensitivity and spectral resolution. We also present a related 13C/15N-F2-coupled homodecoupled version of the CLIP-HSQC experiment for the simultaneous measurement of 1JCH and 1JNH from the simplified doublets observed along the direct dimension. Finally, a novel J-resolved HSQC experiment has been designed for the simple and automated determination of both 1JCH/1JNH from a 2D J-resolved spectrum.
AB - The concepts of pure-shift NMR and time-shared NMR are merged in a single experiment. A 13C/15N time-shared version of the real-time BIRD-based broadband homodecoupled HSQC experiment is described. This time-efficient approach affords simultaneously 1H-13C and 1H-15N pure-shift HSQC spectra in a single acquisition, while achieving substantial gains in both sensitivity and spectral resolution. We also present a related 13C/15N-F2-coupled homodecoupled version of the CLIP-HSQC experiment for the simultaneous measurement of 1JCH and 1JNH from the simplified doublets observed along the direct dimension. Finally, a novel J-resolved HSQC experiment has been designed for the simple and automated determination of both 1JCH/1JNH from a 2D J-resolved spectrum.
KW - HSQC
KW - J-resolved HSQC
KW - NMR
KW - Proton-carbon coupling constants
KW - Proton-nitrogen coupling constants
KW - Pure-shift HSQC
KW - Time-shared NMR
UR - http://www.scopus.com/inward/record.url?scp=85057215300&partnerID=8YFLogxK
U2 - 10.1016/j.jmr.2018.11.005
DO - 10.1016/j.jmr.2018.11.005
M3 - Article
C2 - 30502625
SN - 1090-7807
VL - 298
SP - 23
EP - 30
JO - Journal of Magnetic Resonance
JF - Journal of Magnetic Resonance
ER -