© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim d-Fructose-6-phosphate aldolase (FSA) was probed for extended nucleophile promiscuity by using a series of fluorogenic substrates to reveal retro-aldol activity. Four nucleophiles ethanal, propanone, butanone, and cyclopentanone were subsequently confirmed to be non-natural substrates in the synthesis direction using the wild-type enzyme and its D6H variant. This exceptional widening of the nucleophile substrate scope offers a rapid entry, in good yields and high stereoselectivity, to less oxygenated alkyl ketones and aldehydes, which was hitherto impossible.
|Journal||Chemistry - A European Journal|
|Publication status||Published - 11 Apr 2017|
- fructose-6-phosphate aldolase
- phosphorylated compounds