Brain extracellular fluid protein changes in acute stroke patients

Loïc Dayon; Natacha Turck; Teresa García-Berrocoso; Nadia Walter; Pierre R. Burkhard; Anna Vilalta; Juan Sahuquillo; Joan Montaner; Jean Charles Sanchez

doi:https://doi.org/10.1021/pr101123t

Brain extracellular fluid protein changes in acute stroke patients

Loïc Dayon, Natacha Turck, Teresa García-Berrocoso, Nadia Walter, Pierre R. Burkhard, Anna Vilalta, Juan Sahuquillo, Joan Montaner, Jean Charles Sanchez

Department of Surgery

Research output: Contribution to journal › Article › Research › peer-review

60 Citations (Scopus)

Abstract

In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers. © 2010 American Chemical Society.

Original language	English
Pages (from-to)	1043-1051
Journal	Journal of Proteome Research
Volume	10
DOIs	https://doi.org/10.1021/pr101123t
Publication status	Published - 4 Mar 2011

Keywords

Microdialysis
biomarkers
brain extracellular fluid
cerebrovascular disease
infarct core
ischemic penumbra
microdialysate
peroxiredoxin
proteomics
stroke

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title = "Brain extracellular fluid protein changes in acute stroke patients",

abstract = "In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers. {\textcopyright} 2010 American Chemical Society.",

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Brain extracellular fluid protein changes in acute stroke patients. / Dayon, Loïc; Turck, Natacha; García-Berrocoso, Teresa; Walter, Nadia; Burkhard, Pierre R.; Vilalta, Anna; Sahuquillo, Juan; Montaner, Joan; Sanchez, Jean Charles.

In: Journal of Proteome Research, Vol. 10, 04.03.2011, p. 1043-1051.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Brain extracellular fluid protein changes in acute stroke patients

AU - Dayon, Loïc

AU - Turck, Natacha

AU - García-Berrocoso, Teresa

AU - Walter, Nadia

AU - Burkhard, Pierre R.

AU - Vilalta, Anna

AU - Sahuquillo, Juan

AU - Montaner, Joan

AU - Sanchez, Jean Charles

PY - 2011/3/4

Y1 - 2011/3/4

N2 - In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers. © 2010 American Chemical Society.

AB - In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers. © 2010 American Chemical Society.

KW - Microdialysis

KW - biomarkers

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KW - infarct core

KW - ischemic penumbra

KW - microdialysate

KW - peroxiredoxin

KW - proteomics

KW - stroke

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