TY - JOUR
T1 - Brain-derived neurotrophic factor and its receptors in Bergmann glia cells
AU - Poblete-Naredo, Irais
AU - Guillem, Alain M.
AU - Juárez, Claudia
AU - Zepeda, Rossana C.
AU - Ramírez, Leticia
AU - Caba, Mario
AU - Hernández-Kelly, Luisa C.
AU - Aguilera, José
AU - López-Bayghen, Esther
AU - Ortega, Arturo
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Brain-derived neurotrophic factor is an abundant and widely distributed neurotrophin expressed in the Central Nervous System. It is critically involved in neuronal differentiation and survival. The expression of brain-derived neurotrophic factor and that of its catalytic active cognate receptor (TrkB) has been extensively studied in neuronal cells but their expression and function in glial cells is still controversial. Despite of this fact, brain-derived neurotrophic factor is released from astrocytes upon glutamate stimulation. A suitable model to study glia/neuronal interactions, in the context of glutamatergic synapses, is the well-characterized culture of chick cerebellar Bergmann glia cells. Using, this system, we show here that BDNF and its functional receptor are present in Bergmann glia and that BDNF stimulation is linked to the activation of the phosphatidyl-inositol 3 kinase/protein kinase C/mitogen-activated protein kinase/Activator Protein-1 signaling pathway. Accordingly, reverse transcription-polymerase chain reaction (RT-PCR) experiments predicted the expression of full-length and truncated TrkB isoforms. Our results suggest that Bergmann glia cells are able to express and respond to BDNF stimulation favoring the notion of their pivotal role in neuroprotection. © 2011 Elsevier B.V. All rights reserved.
AB - Brain-derived neurotrophic factor is an abundant and widely distributed neurotrophin expressed in the Central Nervous System. It is critically involved in neuronal differentiation and survival. The expression of brain-derived neurotrophic factor and that of its catalytic active cognate receptor (TrkB) has been extensively studied in neuronal cells but their expression and function in glial cells is still controversial. Despite of this fact, brain-derived neurotrophic factor is released from astrocytes upon glutamate stimulation. A suitable model to study glia/neuronal interactions, in the context of glutamatergic synapses, is the well-characterized culture of chick cerebellar Bergmann glia cells. Using, this system, we show here that BDNF and its functional receptor are present in Bergmann glia and that BDNF stimulation is linked to the activation of the phosphatidyl-inositol 3 kinase/protein kinase C/mitogen-activated protein kinase/Activator Protein-1 signaling pathway. Accordingly, reverse transcription-polymerase chain reaction (RT-PCR) experiments predicted the expression of full-length and truncated TrkB isoforms. Our results suggest that Bergmann glia cells are able to express and respond to BDNF stimulation favoring the notion of their pivotal role in neuroprotection. © 2011 Elsevier B.V. All rights reserved.
KW - BDNF
KW - Bergmann glia
KW - Transcriptional control
U2 - https://doi.org/10.1016/j.neuint.2011.10.002
DO - https://doi.org/10.1016/j.neuint.2011.10.002
M3 - Article
VL - 59
SP - 1133
EP - 1144
JO - Neurochemistry International
JF - Neurochemistry International
SN - 0197-0186
ER -