Blood amyloid beta levels in healthy, mild cognitive impairment and Alzheimer's disease individuals: Replication of diastolic blood pressure correlations and analysis of critical covariates

Agustín Ruiz, Pedro Pesini, Ana Espinosa, Virginia Pérez-Grijalba, Sergi Valero, Oscar Sotolongo-Grau, Montserrat Alegret, Inmaculada Monleón, Asunción Lafuente, Mar Buendía, Marta Ibarria, Susana Ruiz, Isabel Hernández, Itziar San José, Lluís Tárraga, Mercè Boada, Manuel Sarasa

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23 Citations (Scopus)

Abstract

Plasma amyloid beta (Aβ) levels are being investigated as potential biomarkers for Alzheimer's disease. In AB128 cross-sectional study, a number of medical relevant correlates of blood Aβ40 or Aβ42 were analyzed in 140 subjects (51 Alzheimer's disease patients, 53 healthy controls and 36 individuals diagnosed with mild cognitive impairment). We determined the association between multiple variables with Aβ40 and Aβ42 levels measured in three different blood compartments called i) Aβ directly accessible (DA) in the plasma, ii) Aβ recovered from the plasma matrix (RP) after diluting the plasma sample in a formulated buffer, and iii) associated with the remaining cellular pellet (CP). We confirmed that diastolic blood pressure (DBP) is consistently correlated with blood DA Aβ40 levels (r=-0.19, P=0.032). These results were consistent in the three phenotypic groups studied. Importantly, the observation resisted covariation with age, gender or creatinine levels. Observed effect size and direction of Aβ40 levels/DBP correlation are in accordance with previous reports. Of note, DA Aβ40 and the RP Aβ40 were also strongly associated with creatinine levels (r=0.599, P≪0.001) and to a lesser extent to urea, age, hematocrit, uric acid and homocysteine (p<0.001). DBP and the rest of statistical significant correlates identified should be considered as potential confounder factors in studies investigating blood Aβ levels as potential AD biomarker. Remarkably, the factors affecting Aβ levels in plasma (DA, RP) and blood cell compartments (CP) seem completely different. © 2013 Ruiz et al.
Original languageEnglish
Article numbere81334
JournalPLoS ONE
Volume8
Issue number11
DOIs
Publication statusPublished - 27 Nov 2013

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