TY - JOUR
T1 - Biotransformation of chloramphenicol by white-rot-fungi Trametes versicolor under cadmium stress
AU - Tan, Zewen
AU - Losantos, Diana
AU - Li, Yongtao
AU - Sarrà, Montserrat
N1 - Publisher Copyright:
© 2022
PY - 2023/2
Y1 - 2023/2
N2 - The recalcitrant chloramphenicol (CAP) combined with heavy metals cadmium (Cd) commonly co-existed in the environment, posing threat to environment health. The capacity of Trametes versicolor to remove/biodegrade CAP in air-pulse fluidized-bed reactor was evaluated, even under Cd stress. T. versicolor could remove 44 % CAP of 5 mg/L in 15 days, even 51 % CAP under 1 mg/L Cd stress. Sustained Cd stress inhibited CAP biodegradation and Cd removal in a 5-batches of a 5-days cycle sequential batch reactor. Nine transformation products and two novel pathways were proposed, with initial multi-step transformation reaction into CP2 and allylic alcohol, respectively. Furthermore, the main mechanism of Cd removal by T. versicolor was extracellular surface bioadsorption and intracellular accumulation. This study filled the gap of the mechanism of simultaneous CAP removal/biodegradation and Cd removal by white-rot fungi T. versicolor, which offer a theoretical basis for future application of biological removal of CAP containing wastewater.
AB - The recalcitrant chloramphenicol (CAP) combined with heavy metals cadmium (Cd) commonly co-existed in the environment, posing threat to environment health. The capacity of Trametes versicolor to remove/biodegrade CAP in air-pulse fluidized-bed reactor was evaluated, even under Cd stress. T. versicolor could remove 44 % CAP of 5 mg/L in 15 days, even 51 % CAP under 1 mg/L Cd stress. Sustained Cd stress inhibited CAP biodegradation and Cd removal in a 5-batches of a 5-days cycle sequential batch reactor. Nine transformation products and two novel pathways were proposed, with initial multi-step transformation reaction into CP2 and allylic alcohol, respectively. Furthermore, the main mechanism of Cd removal by T. versicolor was extracellular surface bioadsorption and intracellular accumulation. This study filled the gap of the mechanism of simultaneous CAP removal/biodegradation and Cd removal by white-rot fungi T. versicolor, which offer a theoretical basis for future application of biological removal of CAP containing wastewater.
KW - Biotransformation pathway
KW - Cadmium stress
KW - Chloramphenicol biotransformation
KW - Fluidized-bed reactor
KW - Trametes versicolor
UR - http://www.scopus.com/inward/record.url?scp=85144563803&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.biortech.2022.128508
DO - https://doi.org/10.1016/j.biortech.2022.128508
M3 - Article
C2 - 36549514
AN - SCOPUS:85144563803
SN - 0960-8524
VL - 369
JO - Agricultural Wastes
JF - Agricultural Wastes
M1 - 128508
ER -
