Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

Giorgio Mannina, Dario Presti, Gabriela Montiel-Jarillo, María Eugenia Suárez-Ojeda

Research output: Contribution to journalArticleResearch

31 Citations (Scopus)

Abstract

© 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
Original languageEnglish
Pages (from-to)361-369
Number of pages9
JournalBioresource Technology
Volume282
DOIs
Publication statusPublished - 1 Jun 2019

Keywords

  • Bioplastic
  • PHA
  • Wastewater treatment
  • Water resource recovery
  • Bioreactors
  • Polyhydroxyalkanoates/isolation & purification
  • Plastics/isolation & purification
  • Biomass
  • Fermentation
  • Waste Water/chemistry
  • PERFORMANCE
  • BIOMASS
  • POLY(3-HYDROXYBUTYRATE) PRODUCTION
  • ENRICHMENT
  • EFFICIENT
  • MICROSTRUCTURE

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