Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

Giorgio Mannina; Dario Presti; Gabriela Montiel-Jarillo; María Eugenia Suárez-Ojeda

doi:10.1016/j.biortech.2019.03.037

Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

Giorgio Mannina, Dario Presti, Gabriela Montiel-Jarillo, María Eugenia Suárez-Ojeda

Department of Chemical, Biological and Environmental Engineering
Institute of Environmental Science and Technology (ICTA-UAB)
Grup de Tractament Biologic i Valorització d'efluents Liquids i Gasosos. Eliminació de Nutrients, Olors i compostos organics Volatils. GENOCOV

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69 Citations (Scopus)

Abstract

© 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.

Original language	English
Pages (from-to)	361-369
Number of pages	9
Journal	Bioresource Technology
Volume	282
DOIs	https://doi.org/10.1016/j.biortech.2019.03.037
Publication status	Published - 1 Jun 2019

Keywords

Bioplastic
PHA
Wastewater treatment
Water resource recovery
Bioreactors
Polyhydroxyalkanoates/isolation & purification
Plastics/isolation & purification
Biomass
Fermentation
Waste Water/chemistry
PERFORMANCE
BIOMASS
POLY(3-HYDROXYBUTYRATE) PRODUCTION
ENRICHMENT
EFFICIENT
MICROSTRUCTURE

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10.1016/j.biortech.2019.03.037

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title = "Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures",

abstract = "{\textcopyright} 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.",

keywords = "Bioplastic, PHA, Wastewater treatment, Water resource recovery, Bioreactors, Polyhydroxyalkanoates/isolation & purification, Plastics/isolation & purification, Biomass, Fermentation, Waste Water/chemistry, PERFORMANCE, BIOMASS, POLY(3-HYDROXYBUTYRATE) PRODUCTION, ENRICHMENT, EFFICIENT, MICROSTRUCTURE",

author = "Giorgio Mannina and Dario Presti and Gabriela Montiel-Jarillo and Su{\'a}rez-Ojeda, {Mar{\'i}a Eugenia}",

year = "2019",

month = jun,

day = "1",

doi = "10.1016/j.biortech.2019.03.037",

language = "English",

volume = "282",

pages = "361--369",

journal = "Agricultural Wastes",

issn = "0960-8524",

publisher = "Elsevier",

}

TY - JOUR

T1 - Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

AU - Mannina, Giorgio

AU - Presti, Dario

AU - Montiel-Jarillo, Gabriela

AU - Suárez-Ojeda, María Eugenia

PY - 2019/6/1

Y1 - 2019/6/1

N2 - © 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.

AB - © 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.

KW - Bioplastic

KW - PHA

KW - Wastewater treatment

KW - Water resource recovery

KW - Bioreactors

KW - Polyhydroxyalkanoates/isolation & purification

KW - Plastics/isolation & purification

KW - Biomass

KW - Fermentation

KW - Waste Water/chemistry

KW - PERFORMANCE

KW - BIOMASS

KW - POLY(3-HYDROXYBUTYRATE) PRODUCTION

KW - ENRICHMENT

KW - EFFICIENT

KW - MICROSTRUCTURE

UR - http://www.mendeley.com/research/bioplastic-recovery-wastewater-new-protocol-polyhydroxyalkanoates-pha-extraction-mixed-microbial-cul

U2 - 10.1016/j.biortech.2019.03.037

DO - 10.1016/j.biortech.2019.03.037

M3 - Article

C2 - 30884455

SN - 0960-8524

VL - 282

SP - 361

EP - 369

JO - Agricultural Wastes

JF - Agricultural Wastes

ER -

Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

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Keywords

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