TY - CHAP
T1 - Biomedical Applications of Bacterial Inclusion Bodies
AU - Ratera, Imma
AU - Peternel, Spela
AU - Seras-Franzoso, Joaquin
AU - Cano-Garrido, Olivia
AU - García-Fruitós, Elena
AU - Cubarsí, Rafael
AU - Vazquez, Esther
AU - Corchero, José Luis
AU - Rodríguez-Carmona, Escarlata
AU - Veciana, Jaume
AU - Villaverde, Antonio
PY - 2014/4/14
Y1 - 2014/4/14
N2 - © 2014 by John Wiley & Sons, Inc. All rights reserved. The production of recombinant proteins is a major technological platform in biotechnology, pharmacology, and associated disciplines, and a convenient source of polypeptides for the biocatalysis, food, and pharmaceutical industries. Escherichia coli, have been adapted during three decades as cellular factories for protein production. Using these biological platforms, enzymes, hormones, growth factors, cytoquines, antigens, immunogens, and a diversity of supramolecular complexes have been obtained for characterization or to be used as tools in catalysis or biomedicine. Inclusion bodies (IBs) have recently been observed as emerging biomaterials with potential biomedical applications. This has been empowered by the fact that these protein clusters are biologically active and also mechanically stable. Once purified, IBs are regular particles with low polydispersity, usually spherical or ovoid, ranging from 50 to 1000nm in diameter. The potential of bacterial cells to generate therapeutic nanopills should be explored fully in the context of innovative medicines.
AB - © 2014 by John Wiley & Sons, Inc. All rights reserved. The production of recombinant proteins is a major technological platform in biotechnology, pharmacology, and associated disciplines, and a convenient source of polypeptides for the biocatalysis, food, and pharmaceutical industries. Escherichia coli, have been adapted during three decades as cellular factories for protein production. Using these biological platforms, enzymes, hormones, growth factors, cytoquines, antigens, immunogens, and a diversity of supramolecular complexes have been obtained for characterization or to be used as tools in catalysis or biomedicine. Inclusion bodies (IBs) have recently been observed as emerging biomaterials with potential biomedical applications. This has been empowered by the fact that these protein clusters are biologically active and also mechanically stable. Once purified, IBs are regular particles with low polydispersity, usually spherical or ovoid, ranging from 50 to 1000nm in diameter. The potential of bacterial cells to generate therapeutic nanopills should be explored fully in the context of innovative medicines.
KW - Bacterial inclusion bodies
KW - Bacterial inclusion bodies (IBs)
KW - Biomedical applications
KW - Escherichia coli
KW - IB protein
KW - Nanopills
KW - Supramolecular complexes
KW - Tissue engineering
U2 - 10.1002/9781118845363.ch8
DO - 10.1002/9781118845363.ch8
M3 - Chapter
SN - 978-1-118-44852-6
VL - 9781118448526
T3 - Series in Probability and Stattistics
SP - 203
EP - 220
BT - Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells
CY - Hoboken (US)
ER -