Biological imaging using light-addressable potentiometric sensors and scanning photo-induced impedance microscopy

Fan Wu, Inmaculada Campos, De Wen Zhang, Steffi Krause

Research output: Contribution to journalReview articleResearchpeer-review

18 Citations (Scopus)

Abstract

© 2017 The Author(s) Published by the Royal Society. All rights reserved. Light-addressable potentiometric sensors (LAPS) and scanning photo-induced impedance microscopy (SPIM) use photocurrent measurements at electrolyte-insulator-semiconductor substrates for spatiotemporal imaging of electrical potentials and impedance. The techniques have been used for the interrogation of sensor arrays and the imaging of biological systems. Sensor applications range from the detection of different types of ions and the label-free detection of charged molecules such as DNA and proteins to enzyme-based biosensors. Imaging applications include the temporal imaging of extracellular potentials and dynamic concentration changes in microfluidic channels and the lateral imaging of cell surface charges and cell metabolism. This paper will investigate the current state of the art of the measurement technology with a focus on spatial and temporal resolution and review the biological applications, these techniques have been used for. An outlook on future developments in the field will be given.
Original languageEnglish
Article number20170130
JournalProceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences
Volume473
Issue number2201
DOIs
Publication statusPublished - 1 May 2017

Keywords

  • Biosensors
  • Electrochemical imaging, potentiometric sensor
  • Impedance
  • Ion concentrations

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