Biofabrication of functional protein nanoparticles through simple His-tag engineering

Hèctor López-Laguna, Julieta M. Sánchez, José Vicente Carratalá, Mauricio Rojas-Peña, Laura Sánchez-García, Eloi Parladé, Alejandro Sánchez-Chardi, Eric Voltà-Durán, Naroa Serna, Olivia Cano-Garrido, Sandra Flores, Neus Ferrer-Miralles, Verónica Nolan, Ario De Marco, Nerea Roher, Ugutz Unzueta, Esther Vazquez*, Antonio Villaverde

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We have developed a simple, robust, and fully transversal approach for the a-la-carte fabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Zn2+ ions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats.

Original languageEnglish
JournalACS Sustainable Chemistry and Engineering
DOIs
Publication statusAccepted in press - 2021

Keywords

  • Biomaterials design
  • Divalent cations
  • Nanoparticles
  • Protein engineering
  • Protein materials

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