Biodiesel synthesis in a solvent-free system by recombinant Rhizopus oryzae: comparative study between a stirred tank and a packed-bed batch reactor

Albert Canet, KÍrian Bonet-Ragel, M. Dolors Benaiges, Francisco Valero

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

© 2017 Informa UK Limited, trading as Taylor & Francis Group. A simultaneous synthesis of biodiesel, as fatty acid methyl esters, and monoacylglycerols catalysed by the recombinant Rhizopus oryzae lipase immobilized by adsorption on Relizyme OD/403M is presented. The use of this 1(3)-positional specific lipase prevents the formation of glycerol as a by-product, thus avoiding its drawbacks. The synthesis was carried out in a solvent-free system and it has been studied in two different reactor systems: stirred tank and packed-bed reactor. Stirred tank reactor presented a high-initial reaction rate and achieved a 33.6% yield, which corresponds to a value of 50.4% of the maximum yield that can be achieved with a 1(3)-positional specific lipase. In packed-bed reactor there was a smaller initial reaction rate, but it was achieved a 49.1% yield, which corresponds to a 73.6% of the maximum yield. When a second batch is performed, the yield decreased only 4% when packed-bed reactor is employed whereas a drastic decrease is observed in a stirred tank operation. Therefore, packed-bed reactor showed a best performance and minor damage to the biocatalyst.
Original languageEnglish
Pages (from-to)35-40
JournalBiocatalysis and Biotransformation
Volume35
Issue number1
DOIs
Publication statusPublished - 2 Jan 2017

Keywords

  • biodiesel
  • packed-bed reactor
  • Rhizopus oryzae lipase
  • transesterification

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