Biocatalytic synthesis of vanillin by an immobilised eugenol oxidase: High biocatalyst yield by enzyme recycling

Miquel Garcia Bofill, Peter Sutton , Harrie Straatman, Jan Brummund, Martin Schürmann, Marina Guillen Montalban, Gregorio Alvaro Campos

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Vanilla is the second most expensive spice in the world. This issue triggered the necessity to obtain its flavour compound, vanillin, by alternative methods, most of which have focused on biosynthesis since it can be labelled as “natural” and sold at higher price. In this work, a process for the enzymatic synthesis of vanillin has been optimised, not only from a process metrics point of view but also from an environmentally sustainable perspective. The maximum biocatalyst activity and stability was taken into account for the selection of the reaction conditions. Soluble and immobilised eugenol oxidase (EUGO) was tested under both reaction conditions. The optimum conditions obtained lead to an organic solvent free process reaching 9.9 g prod L−1 h−1. Moreover, the use of immobilised EUGO allowed the biocatalyst to be reused up to 18 reaction cycles, improving the biocatalyst yield more than 12-fold, thus also reducing the biocatalyst associated cost
Original languageSpanish
Article number117934
Pages (from-to)1-7
Number of pages7
JournalApplied Catalysis A: General
Volume610
DOIs
Publication statusPublished - 2021

Keywords

  • Bioconversion
  • Biocatalyst
  • Enzymatic biotransformation
  • Eugenol oxidase
  • immobilisation
  • immobilization
  • Natural flavour
  • vanillin synthesis

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