Binding of the antitubercular pro-drug isoniazid in the heme access channel of catalase-peroxidase (KatG). A combined structural and metadynamics investigation

Pietro Vidossich, Peter C. Loewen, Xavi Carpena, Giacomo Fiorin, Ignacio Fita, Carme Rovira

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Abstract

Isonicotinic acid hydrazide (isoniazid or INH) is a front line antitubercular pro-drug that is converted to its active form, isonicotinyl-NAD, by the bacterial catalase-peroxidase KatG. Understanding the role of KatG in the INH activation process has been hampered by a lack of knowledge of the actual drug binding site. In this work, we have investigated the binding of INH in the main access channel of KatG with a combination of molecular dynamics, using an enhanced-sampling technique (metadynamics), X-ray crystallography, and site-directed mutagenesis. The metadynamics simulations show that there are several weak drug binding sites along the access channel. Moreover, the simulations evidence that complete entrance to the heme active site is impeded by an aspartate residue (D141) located above the heme. This has been confirmed by structural and functional analysis of the D141A mutant, leading to the first X-ray crystallography evidence of INH at the heme access channel. © 2014 American Chemical Society.
Original languageEnglish
Pages (from-to)2924-2931
JournalJournal of Physical Chemistry B
Volume118
Issue number11
DOIs
Publication statusPublished - 20 Mar 2014

Fingerprint Dive into the research topics of 'Binding of the antitubercular pro-drug isoniazid in the heme access channel of catalase-peroxidase (KatG). A combined structural and metadynamics investigation'. Together they form a unique fingerprint.

  • Cite this

    Vidossich, P., Loewen, P. C., Carpena, X., Fiorin, G., Fita, I., & Rovira, C. (2014). Binding of the antitubercular pro-drug isoniazid in the heme access channel of catalase-peroxidase (KatG). A combined structural and metadynamics investigation. Journal of Physical Chemistry B, 118(11), 2924-2931. https://doi.org/10.1021/jp4123425