Binding of HMG14 non-histone protein to histones H2A, H2B, H1 and DNA in reconstituted chromatin

Enric Espel; Jordi Bernués; Josep A. Pérez-Pons; Enrique Querol

doi:10.1016/0006-291X(85)91910-2

Binding of HMG14 non-histone protein to histones H2A, H2B, H1 and DNA in reconstituted chromatin

Enric Espel, Jordi Bernués, Josep A. Pérez-Pons, Enrique Querol

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Abstract

The interaction between calf thymus HMG14 and rat liver chromatin components has been studied via reconstitution and chemical cross-linking. Selective labeling of HMG14 with photoactivable reversible heterobifunctional reagents has allowed a clear identification of the histones interacting with it (histones H2A, H2B and H1). These results are not dependent on whether the chromatin samples used were bulk chromatin, mononucleosomes, or core particles (for H2A and H2B). In addition to histone proteins, DNA also seems to be involved in HMG14 attachment to nucleosome. © 1985.

Original language	English
Pages (from-to)	1031-1037
Journal	Biochemical and Biophysical Research Communications
Volume	132
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91910-2
Publication status	Published - 15 Nov 1985

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title = "Binding of HMG14 non-histone protein to histones H2A, H2B, H1 and DNA in reconstituted chromatin",

abstract = "The interaction between calf thymus HMG14 and rat liver chromatin components has been studied via reconstitution and chemical cross-linking. Selective labeling of HMG14 with photoactivable reversible heterobifunctional reagents has allowed a clear identification of the histones interacting with it (histones H2A, H2B and H1). These results are not dependent on whether the chromatin samples used were bulk chromatin, mononucleosomes, or core particles (for H2A and H2B). In addition to histone proteins, DNA also seems to be involved in HMG14 attachment to nucleosome. {\textcopyright} 1985.",

author = "Enric Espel and Jordi Bernu{\'e}s and P{\'e}rez-Pons, {Josep A.} and Enrique Querol",

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T1 - Binding of HMG14 non-histone protein to histones H2A, H2B, H1 and DNA in reconstituted chromatin

AU - Espel, Enric

AU - Bernués, Jordi

AU - Pérez-Pons, Josep A.

AU - Querol, Enrique

PY - 1985/11/15

Y1 - 1985/11/15

N2 - The interaction between calf thymus HMG14 and rat liver chromatin components has been studied via reconstitution and chemical cross-linking. Selective labeling of HMG14 with photoactivable reversible heterobifunctional reagents has allowed a clear identification of the histones interacting with it (histones H2A, H2B and H1). These results are not dependent on whether the chromatin samples used were bulk chromatin, mononucleosomes, or core particles (for H2A and H2B). In addition to histone proteins, DNA also seems to be involved in HMG14 attachment to nucleosome. © 1985.

AB - The interaction between calf thymus HMG14 and rat liver chromatin components has been studied via reconstitution and chemical cross-linking. Selective labeling of HMG14 with photoactivable reversible heterobifunctional reagents has allowed a clear identification of the histones interacting with it (histones H2A, H2B and H1). These results are not dependent on whether the chromatin samples used were bulk chromatin, mononucleosomes, or core particles (for H2A and H2B). In addition to histone proteins, DNA also seems to be involved in HMG14 attachment to nucleosome. © 1985.

U2 - 10.1016/0006-291X(85)91910-2

DO - 10.1016/0006-291X(85)91910-2

M3 - Article

VL - 132

SP - 1031

EP - 1037

IS - 3

ER -

Binding of HMG14 non-histone protein to histones H2A, H2B, H1 and DNA in reconstituted chromatin

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