Beneficial effects of IL-37 after spinal cord injury in mice

Marina Coll-Miró, Isaac Francos-Quijorna, Eva Santos-Nogueira, Abel Torres-Espin, Philip Bufler, Charles A. Dinarello, Rubèn López-Vales

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)

Abstract

IL-37, a member of the IL-1 family, broadly reduces innate inflammation as well as acquired immunity. Whether the antiinflammatory properties of IL-37 extend to the central nervous system remains unknown, however. In the present study, we subjected mice transgenic for human IL-37 (hIL-37tg) and wild-type (WT) mice to spinal cord contusion injury and then treated them with recombinant human IL-37 (rIL-37). In the hIL-37tg mice, the expression of IL-37 was barely detectable in the uninjured cords, but was strongly induced at 24 h and 72 h after the spinal cord injury (SCI). Compared with WT mice, hIL-37tg mice exhibited increased myelin and neuronal sparing and protection against locomotor deficits, including 2.5-fold greater speed in a forced treadmill challenge. Reduced levels of cytokines (e.g., an 80% reduction in IL-6) were observed in the injured cords of hIL-37tg mice, along with lower numbers of blood-borne neutrophils, macrophages, and activated microglia. We treated WT mice with a single intraspinal injection of either full-length or processed rIL-37 after the injury and found that the IL-37-treated mice had significantly enhanced locomotor skills in an open field using the Basso Mouse Scale, as well as supported faster speed on a mechanical treadmill. Treatment with both forms of rIL-37 led to similar beneficial effects on locomotor recovery after SCI. This study presents novel data indicating that IL-37 suppresses inflammation in a clinically relevant model of SCI, and suggests that rIL-37 may have therapeutic potential for the treatment of acute SCI.
Original languageEnglish
Pages (from-to)1411-1416
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
DOIs
Publication statusPublished - 2 Feb 2016

Keywords

  • Cytokines
  • IL-37
  • Inflammation
  • Neuroprotection
  • Spinal cord injury

Fingerprint

Dive into the research topics of 'Beneficial effects of IL-37 after spinal cord injury in mice'. Together they form a unique fingerprint.

Cite this