BCG Substrains Change Their Outermost Surface as a Function of Growth Media

Sandra Guallar-Garrido; Farners Almiñana-Rapún; Víctor Campo-Pérez; Eduard Torrents; Marina Luquin; Esther Julián

doi:10.3390/vaccines10010040

BCG Substrains Change Their Outermost Surface as a Function of Growth Media

Sandra Guallar-Garrido, Farners Almiñana-Rapún, Víctor Campo-Pérez, Eduard Torrents, Marina Luquin, Esther Julián^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

Mycobacterium bovis bacillus Calmette-Guerin (BCG) efficacy as an immunotherapy tool can be influenced by the genetic background or immune status of the treated population and by the BCG substrain used. BCG comprises several substrains with genetic differences that elicit diverse phenotypic characteristics. Moreover, modifications of phenotypic characteristics can be influenced by culture conditions. However, several culture media formulations are used worldwide to produce BCG. To elucidate the influence of growth conditions on BCG characteristics, five different substrains were grown on two culture media, and the lipidic profile and physico-chemical properties were evaluated. Our results show that each BCG substrain displays a variety of lipidic profiles on the outermost surface depending on the growth conditions. These modifications lead to a breadth of hydrophobicity patterns and a different ability to reduce neutral red dye within the same BCG substrain, suggesting the influence of BCG growth conditions on the interaction between BCG cells and host cells.

Original language	English
Article number	40
Number of pages	8
Journal	Vaccines
Volume	10
Issue number	1
DOIs	https://doi.org/10.3390/vaccines10010040
Publication status	Published - 1 Jan 2022

Keywords

Cell wall
Hydrophobicity
Lipid
Neutral red
PDIM
PGL

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/vaccines10010040

https://ddd.uab.cat/record/252236

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abstract = "Mycobacterium bovis bacillus Calmette-Guerin (BCG) efficacy as an immunotherapy tool can be influenced by the genetic background or immune status of the treated population and by the BCG substrain used. BCG comprises several substrains with genetic differences that elicit diverse phenotypic characteristics. Moreover, modifications of phenotypic characteristics can be influenced by culture conditions. However, several culture media formulations are used worldwide to produce BCG. To elucidate the influence of growth conditions on BCG characteristics, five different substrains were grown on two culture media, and the lipidic profile and physico-chemical properties were evaluated. Our results show that each BCG substrain displays a variety of lipidic profiles on the outermost surface depending on the growth conditions. These modifications lead to a breadth of hydrophobicity patterns and a different ability to reduce neutral red dye within the same BCG substrain, suggesting the influence of BCG growth conditions on the interaction between BCG cells and host cells.",

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AU - Guallar-Garrido, Sandra

AU - Almiñana-Rapún, Farners

AU - Campo-Pérez, Víctor

AU - Torrents, Eduard

AU - Luquin, Marina

AU - Julián, Esther

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Mycobacterium bovis bacillus Calmette-Guerin (BCG) efficacy as an immunotherapy tool can be influenced by the genetic background or immune status of the treated population and by the BCG substrain used. BCG comprises several substrains with genetic differences that elicit diverse phenotypic characteristics. Moreover, modifications of phenotypic characteristics can be influenced by culture conditions. However, several culture media formulations are used worldwide to produce BCG. To elucidate the influence of growth conditions on BCG characteristics, five different substrains were grown on two culture media, and the lipidic profile and physico-chemical properties were evaluated. Our results show that each BCG substrain displays a variety of lipidic profiles on the outermost surface depending on the growth conditions. These modifications lead to a breadth of hydrophobicity patterns and a different ability to reduce neutral red dye within the same BCG substrain, suggesting the influence of BCG growth conditions on the interaction between BCG cells and host cells.

AB - Mycobacterium bovis bacillus Calmette-Guerin (BCG) efficacy as an immunotherapy tool can be influenced by the genetic background or immune status of the treated population and by the BCG substrain used. BCG comprises several substrains with genetic differences that elicit diverse phenotypic characteristics. Moreover, modifications of phenotypic characteristics can be influenced by culture conditions. However, several culture media formulations are used worldwide to produce BCG. To elucidate the influence of growth conditions on BCG characteristics, five different substrains were grown on two culture media, and the lipidic profile and physico-chemical properties were evaluated. Our results show that each BCG substrain displays a variety of lipidic profiles on the outermost surface depending on the growth conditions. These modifications lead to a breadth of hydrophobicity patterns and a different ability to reduce neutral red dye within the same BCG substrain, suggesting the influence of BCG growth conditions on the interaction between BCG cells and host cells.

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BCG Substrains Change Their Outermost Surface as a Function of Growth Media

Abstract

Keywords

UN SDGs

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