Bacterial inclusion body purification

Joaquin Seras-Franzoso; Spela Peternel; Olivia Cano-Garrido; Antonio Villaverde; Elena García-Fruitós

doi:10.1007/978-1-4939-2205-5_16

Bacterial inclusion body purification

Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, Elena García-Fruitós

Research output: Chapter in Book › Chapter › Research › peer-review

10 Citations (Scopus)

Abstract

© Springer Science+Business Media New York 2015. All rights are reserved. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

Original language	English
Title of host publication	Insoluble Proteins: Methods and Protocols
Pages	293-305
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-4939-2205-5_16
Publication status	Published - 1 Dec 2014

Keywords

Bacterial cell free
Bacterial inclusion body
Cell disruption
Nanoparticles
Purification

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10.1007/978-1-4939-2205-5_16

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AU - Peternel, Spela

AU - Cano-Garrido, Olivia

AU - Villaverde, Antonio

AU - García-Fruitós, Elena

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KW - Bacterial cell free

KW - Bacterial inclusion body

KW - Cell disruption

KW - Nanoparticles

KW - Purification

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BT - Insoluble Proteins: Methods and Protocols

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Bacterial inclusion body purification

Abstract

Keywords

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