Bacterial inclusion body purification

Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, Elena García-Fruitós

Research output: Chapter in BookChapterResearchpeer-review

9 Citations (Scopus)

Abstract

© Springer Science+Business Media New York 2015. All rights are reserved. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
Original languageEnglish
Title of host publicationInsoluble Proteins: Methods and Protocols
Pages293-305
Number of pages12
DOIs
Publication statusPublished - 1 Dec 2014

Keywords

  • Bacterial cell free
  • Bacterial inclusion body
  • Cell disruption
  • Nanoparticles
  • Purification

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