© Springer Science+Business Media New York 2015. All rights are reserved. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
|Title of host publication||Insoluble Proteins: Methods and Protocols|
|Number of pages||12|
|Publication status||Published - 1 Dec 2014|
- Bacterial cell free
- Bacterial inclusion body
- Cell disruption