Bacterial inclusion body purification

Joaquin Seras-Franzoso; Spela Peternel; Olivia Cano-Garrido; Antonio Villaverde; Elena García-Fruitós

Bacterial inclusion body purification

Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, Elena García-Fruitós

Research output: Contribution to journal › Article › Research › peer-review

Abstract

© Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

Original language	English
Pages (from-to)	293-305
Journal	Methods in Molecular Biology
Volume	1258
Publication status	Published - 1 Jan 2015

Keywords

Bacterial cell free
Bacterial inclusion body
Cell disruption
Nanoparticles
Purification

Cite this

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title = "Bacterial inclusion body purification",

abstract = "{\textcopyright} Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.",

keywords = "Bacterial cell free, Bacterial inclusion body, Cell disruption, Nanoparticles, Purification",

author = "Joaquin Seras-Franzoso and Spela Peternel and Olivia Cano-Garrido and Antonio Villaverde and Elena Garc{\'i}a-Fruit{\'o}s",

year = "2015",

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volume = "1258",

pages = "293--305",

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TY - JOUR

T1 - Bacterial inclusion body purification

AU - Seras-Franzoso, Joaquin

AU - Peternel, Spela

AU - Cano-Garrido, Olivia

AU - Villaverde, Antonio

AU - García-Fruitós, Elena

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

AB - © Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

KW - Bacterial cell free

KW - Bacterial inclusion body

KW - Cell disruption

KW - Nanoparticles

KW - Purification

M3 - Article

VL - 1258

SP - 293

EP - 305

ER -

Bacterial inclusion body purification

Abstract

Keywords

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