Bacterial inclusion body purification

Joaquin Seras-Franzoso; Spela Peternel; Olivia Cano-Garrido; Antonio Villaverde; Elena García-Fruitós

doi:10.1007/978-1-4939-2205-5_16

Bacterial inclusion body purification

Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, Elena García-Fruitós

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

© Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

Original language	English
Pages (from-to)	293-305
Journal	Methods in Molecular Biology
Volume	1258
DOIs	https://doi.org/10.1007/978-1-4939-2205-5_16
Publication status	Published - 1 Jan 2015

Keywords

Bacterial cell free
Bacterial inclusion body
Cell disruption
Nanoparticles
Purification

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10.1007/978-1-4939-2205-5_16

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title = "Bacterial inclusion body purification",

abstract = "{\textcopyright} Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.",

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AU - Cano-Garrido, Olivia

AU - Villaverde, Antonio

AU - García-Fruitós, Elena

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AB - © Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.

KW - Bacterial cell free

KW - Bacterial inclusion body

KW - Cell disruption

KW - Nanoparticles

KW - Purification

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DO - 10.1007/978-1-4939-2205-5_16

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