Abstract
© Springer Science+Business Media New York 2015. Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
Original language | English |
---|---|
Pages (from-to) | 293-305 |
Journal | Methods in Molecular Biology |
Volume | 1258 |
DOIs | |
Publication status | Published - 1 Jan 2015 |
Keywords
- Bacterial cell free
- Bacterial inclusion body
- Cell disruption
- Nanoparticles
- Purification