Bacterial inclusion bodies: Making gold from waste

Elena García-Fruitós; Esther Vázquez; César Díez-Gil; José Luis Corchero; Joaquin Seras-Franzoso; Imma Ratera; Jaume Veciana; Antonio Villaverde

doi:10.1016/j.tibtech.2011.09.003

Bacterial inclusion bodies: Making gold from waste

Elena García-Fruitós, Esther Vázquez, César Díez-Gil, José Luis Corchero, Joaquin Seras-Franzoso, Imma Ratera, Jaume Veciana, Antonio Villaverde

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Abstract

Many protein species produced in recombinant bacteria aggregate as insoluble protein clusters named inclusion bodies (IBs). IBs are discarded from further processing or are eventually used as a pure protein source for in vitro refolding. Although usually considered as waste byproducts of protein production, recent insights into the physiology of recombinant bacteria and the molecular architecture of IBs have revealed that these protein particles are unexpected functional materials. In this Opinion article, we present the relevant mechanical properties of IBs and discuss the ways in which they can be explored as biocompatible nanostructured materials, mainly, but not exclusively, in biocatalysis and tissue engineering. © 2011 Elsevier Ltd.

Original language	English
Pages (from-to)	65-70
Journal	Trends in Biotechnology
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/j.tibtech.2011.09.003
Publication status	Published - 1 Feb 2012

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AU - García-Fruitós, Elena

AU - Vázquez, Esther

AU - Díez-Gil, César

AU - Corchero, José Luis

AU - Seras-Franzoso, Joaquin

AU - Ratera, Imma

AU - Veciana, Jaume

AU - Villaverde, Antonio

N1 - Elena García-Fruitós*, Esther Vazquez*, César Diez-Gil, José Luis Corchero, Joaquin Seras-Franzoso, Imma Ratera, Jaume Veciana, Antonio Villaverde. (*These authors equally contributed to this work.)

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N2 - Many protein species produced in recombinant bacteria aggregate as insoluble protein clusters named inclusion bodies (IBs). IBs are discarded from further processing or are eventually used as a pure protein source for in vitro refolding. Although usually considered as waste byproducts of protein production, recent insights into the physiology of recombinant bacteria and the molecular architecture of IBs have revealed that these protein particles are unexpected functional materials. In this Opinion article, we present the relevant mechanical properties of IBs and discuss the ways in which they can be explored as biocompatible nanostructured materials, mainly, but not exclusively, in biocatalysis and tissue engineering. © 2011 Elsevier Ltd.

AB - Many protein species produced in recombinant bacteria aggregate as insoluble protein clusters named inclusion bodies (IBs). IBs are discarded from further processing or are eventually used as a pure protein source for in vitro refolding. Although usually considered as waste byproducts of protein production, recent insights into the physiology of recombinant bacteria and the molecular architecture of IBs have revealed that these protein particles are unexpected functional materials. In this Opinion article, we present the relevant mechanical properties of IBs and discuss the ways in which they can be explored as biocompatible nanostructured materials, mainly, but not exclusively, in biocatalysis and tissue engineering. © 2011 Elsevier Ltd.

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Bacterial inclusion bodies: Making gold from waste

Abstract

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