Autoregulation and kinetics of induction of the Rhizobium phaseoli recA gene

Antonio R. Fernández de Henestrosa; Jordi Barbé

doi:10.1016/0027-5107(94)90202-X

Autoregulation and kinetics of induction of the Rhizobium phaseoli recA gene

Antonio R. Fernández de Henestrosa, Jordi Barbé

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

A fusion between the recA gene of Rhizobium phaseoli and the lacZ gene was constructed in vitro and cloned in a mini-Tn5 transposon derivative to obtain chromosomal insertions which make it possible to quantitatively examine their transcriptional regulation in both R. phaseoli and E. coli. Likewise and by insertion of a spectinomycin-resistance gene cassette into the recA gene of R. phaseoli and subsequent maker exchange, a RecA- derivative of this bacterial species has been obtained. Analysis of this recA-lacZ fusion showed that it was inducible by DNA damage in the RecA+ strain of R. phaseoli but not in the RecA- mutant. On the other hand, the recA-lacZ fusion of R. phaseoli was not induced in DNA-damaged RecA+ cells of E. coli. Futhermore, the range of UV doses which give rise to dose dependence in the induction of its respective recA genes is different in R. phaseoli from that in E. coli. © 1994.

Original language	English
Pages (from-to)	99-107
Journal	Mutation Research Regular Papers
Volume	308
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(94)90202-X
Publication status	Published - 1 Jul 1994

Keywords

Non-canonical SOS box
RecA gene induction
Rhizobium phaseoli

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title = "Autoregulation and kinetics of induction of the Rhizobium phaseoli recA gene",

abstract = "A fusion between the recA gene of Rhizobium phaseoli and the lacZ gene was constructed in vitro and cloned in a mini-Tn5 transposon derivative to obtain chromosomal insertions which make it possible to quantitatively examine their transcriptional regulation in both R. phaseoli and E. coli. Likewise and by insertion of a spectinomycin-resistance gene cassette into the recA gene of R. phaseoli and subsequent maker exchange, a RecA- derivative of this bacterial species has been obtained. Analysis of this recA-lacZ fusion showed that it was inducible by DNA damage in the RecA+ strain of R. phaseoli but not in the RecA- mutant. On the other hand, the recA-lacZ fusion of R. phaseoli was not induced in DNA-damaged RecA+ cells of E. coli. Futhermore, the range of UV doses which give rise to dose dependence in the induction of its respective recA genes is different in R. phaseoli from that in E. coli. {\textcopyright} 1994.",

keywords = "Non-canonical SOS box, RecA gene induction, Rhizobium phaseoli",

author = "{Fern{\'a}ndez de Henestrosa}, {Antonio R.} and Jordi Barb{\'e}",

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T1 - Autoregulation and kinetics of induction of the Rhizobium phaseoli recA gene

AU - Fernández de Henestrosa, Antonio R.

AU - Barbé, Jordi

PY - 1994/7/1

Y1 - 1994/7/1

N2 - A fusion between the recA gene of Rhizobium phaseoli and the lacZ gene was constructed in vitro and cloned in a mini-Tn5 transposon derivative to obtain chromosomal insertions which make it possible to quantitatively examine their transcriptional regulation in both R. phaseoli and E. coli. Likewise and by insertion of a spectinomycin-resistance gene cassette into the recA gene of R. phaseoli and subsequent maker exchange, a RecA- derivative of this bacterial species has been obtained. Analysis of this recA-lacZ fusion showed that it was inducible by DNA damage in the RecA+ strain of R. phaseoli but not in the RecA- mutant. On the other hand, the recA-lacZ fusion of R. phaseoli was not induced in DNA-damaged RecA+ cells of E. coli. Futhermore, the range of UV doses which give rise to dose dependence in the induction of its respective recA genes is different in R. phaseoli from that in E. coli. © 1994.

AB - A fusion between the recA gene of Rhizobium phaseoli and the lacZ gene was constructed in vitro and cloned in a mini-Tn5 transposon derivative to obtain chromosomal insertions which make it possible to quantitatively examine their transcriptional regulation in both R. phaseoli and E. coli. Likewise and by insertion of a spectinomycin-resistance gene cassette into the recA gene of R. phaseoli and subsequent maker exchange, a RecA- derivative of this bacterial species has been obtained. Analysis of this recA-lacZ fusion showed that it was inducible by DNA damage in the RecA+ strain of R. phaseoli but not in the RecA- mutant. On the other hand, the recA-lacZ fusion of R. phaseoli was not induced in DNA-damaged RecA+ cells of E. coli. Futhermore, the range of UV doses which give rise to dose dependence in the induction of its respective recA genes is different in R. phaseoli from that in E. coli. © 1994.

KW - Non-canonical SOS box

KW - RecA gene induction

KW - Rhizobium phaseoli

U2 - 10.1016/0027-5107(94)90202-X

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