Objective To determine attitudes to the care of People Living with Human Immunodeficiency Virus (PLHIV) among students and faculty members in a school of nursing in Barcelona. Material and methods An observational, descriptive, cross-sectional study was conducted in faculty and students in a school of nursing in Barcelona (Spain). Data were collected between January and March 2014, through a validated, self-administered online questionnaire (EASE scale). All participants provided their informed consent. Results Of 392 questionnaires sent responses were obtained from 204 participants. Incomplete responses were eliminated leaving 186 completed questionnaires (139 students and 47 faculty members). The overall response rate was 47.4% (45.7% students; 53.4% faculty). A high percentage of positive attitudes was found throughout the sample, particularly in fourth-year students and faculty members (80% and 79.8%, respectively). The lowest percentage of positive attitudes was found in second-year students (70.9%). The highest percentage of positive attitudes was significantly associated (p = 0.045) with a lack of religious beliefs. Conclusions Attitudes to the care of PLHIV among nursing students and faculty members were mainly positive. Some fears and misconceptions mainly concerning fear of infection and beliefs about transmission routes were found in both collectives.