Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes

Leif Hove-Madsen, Anna Llach, Antoni Bayes-Genís, Santiago Roura, Enrique Rodriguez Font, Alejandro Arís, Juan Cinca

Research output: Contribution to journalArticleResearchpeer-review

211 Citations (Scopus)

Abstract

Background-Spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) can generate afterdepolarizations, and these have the potential to initiate arrhythmias. Therefore, an association may exist between spontaneous SR Ca2+ release and initiation of atrial fibrillation (AF), but this has not yet been reported. Methods and Results-Spontaneous Ca2+ release from the SR, manifested as Ca2+ sparks and Ca2+ waves, was recorded with confocal microscopy in atrial myocytes isolated from patients with and those without AF. In addition, the spontaneous inward current associated with Ca2+ waves was measured with the use of the perforated patch-clamp technique. The Ca2+ spark frequency was higher in 8 patients with AF than in 16 patients without (6.0±1.2 versus 2.8±0.8 sparks/mm per second, P<0.05). Similarly, the spontaneous Ca2+ wave frequency was greater in patients with AF (2.8±0.5 versus 1.1±0.3 waves/mm per second, P<0.01). The spontaneous inward current frequency was also higher in 10 patients with AF than in 13 patients without this arrhythmia 0.101±0.028 versus 0.031±0.007 per second, P<0.05, at a clamped potential of -80 mV). In contrast, both the Ca 2+ released from the SR and the Na+-Ca2+ exchange rate induced by a rapid caffeine application were comparable in patients with and without AF. Conclusions-The observed increase in spontaneous Ca2+ release in patients with AF probably is due to an upregulation of the SR Ca2+ release channel activity, which may contribute to the development of AF.
Original languageEnglish
Pages (from-to)1358-1363
JournalCirculation
Volume110
DOIs
Publication statusPublished - 14 Sep 2004

Keywords

  • Arrhythmia
  • Calcium
  • Electrophysiology
  • Ion channels
  • Sarcoplasmic reticulum

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