TY - JOUR
T1 - Association of exposure to second-hand smoke during childhood with blood DNA methylation
AU - Cosin-Tomas, Marta
AU - Hoang, Thanh
AU - Qi, Cancan
AU - Monasso, Giulietta S.
AU - Langdon, Ryan
AU - Kebede Merid, Simon
AU - Calas, Lucinda
AU - de Prado-Bert, Paula
AU - Richmond, Rebecca
AU - Jaddoe, Vincent VW
AU - Duijts, Liesbeth
AU - Wright, John
AU - Annesi-Maesano, Isabella
AU - Grazuleviciene, Regina
AU - Karachaliou, Marianna
AU - Koppelman, Gerard H.
AU - Melén, Erik
AU - Gruzieva, Olena
AU - Vrijheid, Martine
AU - Yousefi, Paul
AU - Felix, Janine F.
AU - London, Stephanie J.
AU - Bustamante, Mariona
N1 - Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.
PY - 2025/1
Y1 - 2025/1
N2 - Introduction: By recent estimates, 40% of children worldwide are exposed to second-hand smoke (SHS), which has been associated with adverse health outcomes. While numerous studies have linked maternal smoking during pregnancy (MSDP) to widespread differences in child blood DNA methylation (DNAm), research specifically examining postnatal SHS exposure remains sparse. To address this gap, we conducted epigenome-wide meta-analyses to identify associations of postnatal SHS and child blood DNAm. Methods: Six cohorts from the Pregnancy And Childhood Epigenetics (PACE) Consortium (total N = 2,695), with SHS data and child blood DNAm (aged 7–9 years) measured with the Illumina 450K array were included in the meta-analysis. Linear regression models adjusted for covariates were fitted to examine the association between the number of household smokers in postnatal life (0, 1, 2+) and child blood DNAm. Sensitivity models without adjusting for MSDP and restricted to mothers who did not smoke during pregnancy were evaluated. Results: Our analysis revealed significant associations (False Discovery Rate < 0.05) between household postnatal SHS exposure and DNAm at 11 CpGs in exposed children. Nine CpGs were mapped to genes (MYO1G, FAM184B, CTDSPL2, LTBP3, PDE10A, and FIBCD1), while 2 CpGs were located in open sea regions. Notably, all except 2 CpGs (mapped to FIBCD1 and CTDSPL2) have previously been linked to either personal smoking habits or in utero exposure to smoking. The models restricted to non-smoking mothers provided similar results. Importantly, several of these CpGs and their associated genes are implicated in conditions exacerbated by or directly linked to SHS. Conclusions: Our findings highlight the potential biological effects of SHS on blood DNAm. These findings support further research on epigenetic factors mediating deleterious effects of SHS on child health and call for public health policies aimed at reducing exposure, particularly in environments where children are present.
AB - Introduction: By recent estimates, 40% of children worldwide are exposed to second-hand smoke (SHS), which has been associated with adverse health outcomes. While numerous studies have linked maternal smoking during pregnancy (MSDP) to widespread differences in child blood DNA methylation (DNAm), research specifically examining postnatal SHS exposure remains sparse. To address this gap, we conducted epigenome-wide meta-analyses to identify associations of postnatal SHS and child blood DNAm. Methods: Six cohorts from the Pregnancy And Childhood Epigenetics (PACE) Consortium (total N = 2,695), with SHS data and child blood DNAm (aged 7–9 years) measured with the Illumina 450K array were included in the meta-analysis. Linear regression models adjusted for covariates were fitted to examine the association between the number of household smokers in postnatal life (0, 1, 2+) and child blood DNAm. Sensitivity models without adjusting for MSDP and restricted to mothers who did not smoke during pregnancy were evaluated. Results: Our analysis revealed significant associations (False Discovery Rate < 0.05) between household postnatal SHS exposure and DNAm at 11 CpGs in exposed children. Nine CpGs were mapped to genes (MYO1G, FAM184B, CTDSPL2, LTBP3, PDE10A, and FIBCD1), while 2 CpGs were located in open sea regions. Notably, all except 2 CpGs (mapped to FIBCD1 and CTDSPL2) have previously been linked to either personal smoking habits or in utero exposure to smoking. The models restricted to non-smoking mothers provided similar results. Importantly, several of these CpGs and their associated genes are implicated in conditions exacerbated by or directly linked to SHS. Conclusions: Our findings highlight the potential biological effects of SHS on blood DNAm. These findings support further research on epigenetic factors mediating deleterious effects of SHS on child health and call for public health policies aimed at reducing exposure, particularly in environments where children are present.
KW - 450K array
KW - Children
KW - DNA methylation
KW - Maternal smoking during pregnancy
KW - Passive smoking
KW - Postnatal exposure
KW - blood DNA methylation
KW - Prenatal Exposure Delayed Effects
KW - Maternal Exposure/statistics & numerical data
KW - Epigenesis, Genetic
KW - Humans
KW - Male
KW - Tobacco Smoke Pollution/adverse effects
KW - Environmental Exposure/statistics & numerical data
KW - Pregnancy
KW - DNA Methylation
KW - Female
KW - Child
KW - Cohort Studies
UR - http://www.scopus.com/inward/record.url?scp=85212070760&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/fd7a7984-6c9e-3cc8-bab5-bebc5bf639cd/
UR - https://portalrecerca.uab.cat/en/publications/78378f22-5d6f-4f2e-a459-478dc8d5e10a
U2 - 10.1016/j.envint.2024.109204
DO - 10.1016/j.envint.2024.109204
M3 - Article
C2 - 39693780
SN - 0160-4120
VL - 195
JO - Environment international
JF - Environment international
M1 - 109204
ER -