Assisted oocyte activation effects on the morphokinetic pattern of derived embryos

Meritxell Martínez, Mercè Durban, Josep Santalo Pedro, Ana Rodríguez, Rita Vassena*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Objective: Assisted oocyte activation (AOA) can restore fertilization rates after IVF/ICSI cycles with fertilization failure. AOA is an experimental technique, and its downstream effects remain poorly characterized. Clarifying the relationship between AOA and embryo, morphokinetics could offer complementary insights into the quality and viability of the embryos obtained with this technique. The aim of this study is to compare the preimplantation morphokinetic development of embryos derived from ICSI-AOA (experimental group) vs. ICSI cycles (control group). Methods: A retrospective cohort study was carried out with 141 embryos from fresh oocyte donation cycles performed between 2013 and 2017; 41 embryos were derived from 7 ICSI-AOA cycles and 100 embryos from 18 ICSI cycles. Morphokinetic development of all embryos was followed using a time-lapse system. Results: We show that embryos from both groups develop similarly for most milestones, with the exception of the time of second polar body extrusion (tPB2) and the time to second cell division (t3). Conclusions: We conclude that ionomycin mediated AOA does not seem to affect the morphokinetic pattern of preimplantation embryo development, despite the alterations found in tPB2 and t3, which could directly reflect the use of a Ca 2+ ionophore as a transient and quick non-physiologic increase of free intracytoplasmic Ca 2+.

Original languageEnglish
Pages (from-to)531-537
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 2021

Keywords

  • AOA
  • ICSI
  • Ionomycin
  • Oocyte donation
  • Time-lapse
  • Humans
  • Time-Lapse Imaging
  • Polar Bodies/metabolism
  • Reproductive Techniques, Assisted
  • Pregnancy
  • Sperm Injections, Intracytoplasmic
  • Fertilization in Vitro
  • Oocytes/growth & development
  • Embryo Transfer
  • Embryonic Development/genetics
  • Adult
  • Female
  • Oocyte Donation
  • Pregnancy Rate
  • FERTILIZATION FAILURE
  • CHILDREN BORN
  • INTRACYTOPLASMIC SPERM INJECTION
  • CALCIUM IONOPHORE
  • OSCILLATIONS
  • MORPHOLOGY
  • PROGRAM

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