Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

M. Espiño; V. Abraira; R. Arroyo; L. Bau; C. Cámara; L. Campos-Ruiz; B. Casanova; C. Espejo; O. Fernández; A. García-Merino; M. I. García-Sánchez; M. Gómez; A. Gosis; G. Izquierdo; J. Meca; X. Montalban; F. Morandeira; J. Olascoaga; A. Prada; E. Quintana; Ll Ramió-Torrentà; A. Rodríguez-Antigüedad; G. Salgado; J. L. Santiago; E. Sarasola; M. Simó-Castelló; J. C. Alvarez-Cermeño; L. M. Villar

doi:10.1016/j.cca.2014.08.004

Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

M. Espiño, V. Abraira, R. Arroyo, L. Bau, C. Cámara, L. Campos-Ruiz, B. Casanova, C. Espejo, O. Fernández, A. García-Merino, M. I. García-Sánchez, M. Gómez, A. Gosis, G. Izquierdo, J. Meca, X. Montalban, F. Morandeira, J. Olascoaga, A. Prada, E. QuintanaLl Ramió-Torrentà, A. Rodríguez-Antigüedad, G. Salgado, J. L. Santiago, E. Sarasola, M. Simó-Castelló, J. C. Alvarez-Cermeño, L. M. Villar

Department of Medicine

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. © 2014 Elsevier B.V.

Original language	English
Pages (from-to)	67-69
Journal	Clinica Chimica Acta
Volume	438
DOIs	https://doi.org/10.1016/j.cca.2014.08.004
Publication status	Published - 1 Jan 2015

Keywords

Biomarker
Immunoglobulin IgM
Multiple sclerosis
Oligoclonal bands

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10.1016/j.cca.2014.08.004

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Espiño, M., Abraira, V., Arroyo, R., Bau, L., Cámara, C., Campos-Ruiz, L., Casanova, B., Espejo, C., Fernández, O., García-Merino, A., García-Sánchez, M. I., Gómez, M., Gosis, A., Izquierdo, G., Meca, J., Montalban, X., Morandeira, F., Olascoaga, J., Prada, A., ... Villar, L. M. (2015). Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice. Clinica Chimica Acta, 438, 67-69. https://doi.org/10.1016/j.cca.2014.08.004

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title = "Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice",

abstract = "Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. {\textcopyright} 2014 Elsevier B.V.",

keywords = "Biomarker, Immunoglobulin IgM, Multiple sclerosis, Oligoclonal bands",

author = "M. Espi{\~n}o and V. Abraira and R. Arroyo and L. Bau and C. C{\'a}mara and L. Campos-Ruiz and B. Casanova and C. Espejo and O. Fern{\'a}ndez and A. Garc{\'i}a-Merino and Garc{\'i}a-S{\'a}nchez, {M. I.} and M. G{\'o}mez and A. Gosis and G. Izquierdo and J. Meca and X. Montalban and F. Morandeira and J. Olascoaga and A. Prada and E. Quintana and Ll Rami{\'o}-Torrent{\`a} and A. Rodr{\'i}guez-Antig{\"u}edad and G. Salgado and Santiago, {J. L.} and E. Sarasola and M. Sim{\'o}-Castell{\'o} and Alvarez-Cerme{\~n}o, {J. C.} and Villar, {L. M.}",

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Espiño, M, Abraira, V, Arroyo, R, Bau, L, Cámara, C, Campos-Ruiz, L, Casanova, B, Espejo, C, Fernández, O, García-Merino, A, García-Sánchez, MI, Gómez, M, Gosis, A, Izquierdo, G, Meca, J, Montalban, X, Morandeira, F, Olascoaga, J, Prada, A, Quintana, E, Ramió-Torrentà, L, Rodríguez-Antigüedad, A, Salgado, G, Santiago, JL, Sarasola, E, Simó-Castelló, M, Alvarez-Cermeño, JC & Villar, LM 2015, 'Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice', Clinica Chimica Acta, vol. 438, pp. 67-69. https://doi.org/10.1016/j.cca.2014.08.004

TY - JOUR

T1 - Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

AU - Espiño, M.

AU - Abraira, V.

AU - Arroyo, R.

AU - Bau, L.

AU - Cámara, C.

AU - Campos-Ruiz, L.

AU - Casanova, B.

AU - Espejo, C.

AU - Fernández, O.

AU - García-Merino, A.

AU - García-Sánchez, M. I.

AU - Gómez, M.

AU - Gosis, A.

AU - Izquierdo, G.

AU - Meca, J.

AU - Montalban, X.

AU - Morandeira, F.

AU - Olascoaga, J.

AU - Prada, A.

AU - Quintana, E.

AU - Ramió-Torrentà, Ll

AU - Rodríguez-Antigüedad, A.

AU - Salgado, G.

AU - Santiago, J. L.

AU - Sarasola, E.

AU - Simó-Castelló, M.

AU - Alvarez-Cermeño, J. C.

AU - Villar, L. M.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. © 2014 Elsevier B.V.

AB - Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. © 2014 Elsevier B.V.

KW - Biomarker

KW - Immunoglobulin IgM

KW - Multiple sclerosis

KW - Oligoclonal bands

U2 - 10.1016/j.cca.2014.08.004

DO - 10.1016/j.cca.2014.08.004

M3 - Article

SN - 0009-8981

VL - 438

SP - 67

EP - 69

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

Abstract

Keywords

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