Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

M. Espiño, V. Abraira, R. Arroyo, L. Bau, C. Cámara, L. Campos-Ruiz, B. Casanova, C. Espejo, O. Fernández, A. García-Merino, M. I. García-Sánchez, M. Gómez, A. Gosis, G. Izquierdo, J. Meca, X. Montalban, F. Morandeira, J. Olascoaga, A. Prada, E. QuintanaLl Ramió-Torrentà, A. Rodríguez-Antigüedad, G. Salgado, J. L. Santiago, E. Sarasola, M. Simó-Castelló, J. C. Alvarez-Cermeño, L. M. Villar

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9 Citations (Scopus)

Abstract

Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice. Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre. Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71. Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. © 2014 Elsevier B.V.
Original languageEnglish
Pages (from-to)67-69
JournalClinica Chimica Acta
Volume438
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Biomarker
  • Immunoglobulin IgM
  • Multiple sclerosis
  • Oligoclonal bands

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